Kracoff-Sella Sharon, Goldfracht Idit, Silverstein Asaf, Landau Shira, Debbi Lior, Beckerman Rita, Shoyhat Hagit, Herman-Bachinsky Yifat, Guterman-Ram Gali, Michael Inbal, Shuhmaher Rita, Zavin Janette, Ben Horin Ronen, Egozi Dana, Levenberg Shulamit
Levenberg Lab, The Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
Plastic Surgery Department, Kaplan Medical Center, Rehovot, Israel.
Biofabrication. 2025 Feb 17;17(2). doi: 10.1088/1758-5090/adaf5a.
Best cosmetic outcomes of breast reconstruction using tissue engineering techniques rely on the scaffold architecture and material, which are currently both to be determined. This study suggests an approach for a rational design of breast-shaped scaffold architecture, in which structural analysis is implemented to predict its stiffness and adjust it to that of the native tissue. This approach can help achieve the goal of optimal scaffold architecture for breast tissue engineering. Based on specifications defined in a preliminary implantation study of a non-rationally designed scaffold, and using analytical modeling and finite element analysis, we rationally designed a polycaprolactone made, 3D-printed, highly porous, breast-shaped scaffold with a stiffness similar to the breast adipose tissue. This scaffold had an architecture of a double-shelled dome connected by pillars, with no bottom to allow direct contact of its fat graft with the host's blood vessels (shelled hemisphere adaptive design (SHAD)). To demonstrate the potential of the SHAD scaffold in breast tissue engineering, a proof-of-concept study was performed, in which SHAD scaffolds were embedded with human adipose derived mesenchymal stem cells, isolated from lipoaspirates, and implanted in nod-scid-gamma mouse model with a delayed fat graft injection. After 4 weeks of implantation, the SHAD implants were vascularized with a viable fat graft, indicating the suitability of the SHAD scaffold for breast tissue engineering.
利用组织工程技术进行乳房重建的最佳美容效果取决于支架结构和材料，目前这两者都有待确定。本研究提出了一种合理设计乳房形状支架结构的方法，其中通过结构分析来预测其刚度并将其调整至与天然组织的刚度相匹配。这种方法有助于实现乳房组织工程中最佳支架结构的目标。基于对非合理设计支架的初步植入研究中所定义的规格，并使用解析建模和有限元分析，我们合理设计了一种由聚己内酯制成的、3D打印的、高度多孔的、乳房形状的支架，其刚度与乳房脂肪组织相似。该支架具有由支柱连接的双壳穹顶结构，没有底部，以便其脂肪移植物能直接与宿主血管接触（带壳半球自适应设计（SHAD））。为了证明SHAD支架在乳房组织工程中的潜力，进行了一项概念验证研究，其中将SHAD支架植入从抽脂物中分离出的人脂肪来源间充质干细胞，并在延迟脂肪移植物注射的情况下植入无胸腺裸鼠-γ模型。植入4周后，SHAD植入物实现了血管化并带有存活的脂肪移植物，表明SHAD支架适用于乳房组织工程。
