Ge Yan, Wang Mingjing, Zhou Yang, Pan Qiyuan, Duan Mengna, Zhang Xiao, Wang Ruolin, Huo Yaru, Guo Yifan, Shen Wei, Liu Min
Department of Oral Mucosal Diseases & Oral Emergency, Hospital of Stomatology, Jilin University, Changchun, China; Jilin Provincial Key Laboratory of Oral Biomedical Engineering, Hospital of Stomatology, Jilin University, Changchun, China.
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.
Colloids Surf B Biointerfaces. 2025 May;249:114535. doi: 10.1016/j.colsurfb.2025.114535. Epub 2025 Jan 24.
Oral ulcers are prone to recurrence and often complicated by bacterial infections. Currently, antibiotics, glucocorticoids, and anesthetics are commonly employed in clinical practice to alleviate symptoms. However, these medications exhibit limited retention in the moist and dynamic environment of the oral cavity, and their long-term use may lead to various side effects or drug resistance. In this study, we designed a borate ester cross-linked oxidation-responsive hydrogel (AHQP hydrogel) for the treatment of oral ulcers. The AHQP hydrogel is composed of phenylboronic acid-functionalized hyaluronic acid, sodium alginate, and quaternized chitosan (QCS), and is further enhanced by the incorporation of proanthocyanidins (PA) to strengthen its borate ester cross-linked network. These natural raw materials comprising the AHQP hydrogel exhibit excellent biocompatibility and degradability, ensuring that the hydrogel is safe for use in the oral cavity. Once the AHQP hydrogel adheres to the wet wound tissue, it degrades in response to reactive oxygen species, thereby releasing QCS and PA to exert therapeutic effects. Our results demonstrated that the AHQP hydrogel exhibits significant antioxidant, anti-inflammatory, antibacterial, and pro-angiogenic properties, thereby facilitating the healing of oral ulcers. Overall, this borate ester cross-linked polysaccharide hydrogel reinforced by PA presents promising prospects for the treatment of oral ulcers.
口腔溃疡容易复发，且常伴有细菌感染。目前，抗生素、糖皮质激素和麻醉剂在临床实践中常用于缓解症状。然而，这些药物在口腔潮湿且动态的环境中滞留时间有限，长期使用可能会导致各种副作用或耐药性。在本研究中，我们设计了一种硼酸酯交联的氧化响应水凝胶（AHQP水凝胶）用于治疗口腔溃疡。AHQP水凝胶由苯基硼酸功能化的透明质酸、海藻酸钠和季铵化壳聚糖（QCS）组成，并通过加入原花青素（PA）进一步增强其硼酸酯交联网络。构成AHQP水凝胶的这些天然原料具有优异的生物相容性和可降解性，确保该水凝胶在口腔中使用安全。一旦AHQP水凝胶粘附在湿润的伤口组织上，它会对活性氧作出响应而降解，从而释放QCS和PA发挥治疗作用。我们的结果表明，AHQP水凝胶具有显著的抗氧化、抗炎、抗菌和促血管生成特性，可以促进口腔溃疡的愈合。总体而言，这种由PA增强的硼酸酯交联多糖水凝胶在口腔溃疡治疗方面具有广阔前景。
