作为胆管癌早期诊断潜在分子标志物的源自胆汁的合胞素-1和溶质载体家族7成员11的循环游离DNA
Bile-Derived cfDNA of Syncytin-1 and SLC7A11 as a Potential Molecular Marker for Early Diagnosis of Cholangiocarcinoma.
作者信息
He Jing, Qian Jingrong, Li Xin, Zhao Xiaoyue, Meng Weiwei, Zhuang Xuewei
机构信息
Second Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
Department of Clinical Laboratory, Shandong Provincial Third Hospital, Shandong University, Jinan, Shandong, China.
出版信息
J Gastrointest Cancer. 2025 Jan 28;56(1):55. doi: 10.1007/s12029-025-01180-5.
PURPOSE
Liquid biopsy technology has received widespread attention in the early diagnosis of cholangiocarcinoma (CCA).
METHODS
We collected bile samples from 48 patients with CCA and 48 patients with gallstones at Shandong Provincial third Hospital. We quantified bile circulating free DNA (cfDNA) of syncytin-1 and SLC7A11, calculated the correlation between syncytin-1 and SLC7A11 expression and clinical parameters by Spearman rank correlation, plotted Receiver Operating Characteristic (ROC) curves, and compared the Area Under Curve (AUC) values to explored early diagnostic utility in patients.
RESULTS
We first found the bile cfDNA of syncytin-1 and SLC7A11 levels in CCA were higher than in gallstones (3.06 vs. 1.32, p < 0.001; 2.39 vs. 1.30, p < 0.001). And there was significant correlation between syncytin-1 and SLC7A11 expression (p = 0.025). Additionally, bile cfDNA of syncytin-1 or SLC7A11 was differentially expressed in gallstones, cholangiocarcinoma stage I-II, and cholangiocarcinoma stage III-IV (p < 0.001; p < 0.001). The AUC of bile cfDNA of syncytin-1 was 0.805 (p < 0.001, specificity of 87.0%), the AUC of bile cfDNA of SLC7A11 was 0.755 (p < 0.001, specificity of 80.4%), and combination of bile cDNA of syncytin-1/SLC7A11/CA19-9 markers improved diagnostic efficiency in CCA patients (AUC: 0.927, p < 0.001).
CONCLUSION
The bile cfDNA of syncytin-1 and SLC7A11 was high expression in cholangiocarcinoma, which may be used as a novel biomarker for early diagnosis.
目的
液体活检技术在胆管癌（CCA）的早期诊断中受到广泛关注。
方法
我们在山东省立第三医院收集了48例CCA患者和48例胆结石患者的胆汁样本。我们对合胞素-1和溶质载体家族7成员11（SLC7A11）的胆汁循环游离DNA（cfDNA）进行定量，通过Spearman等级相关性计算合胞素-1和SLC7A11表达与临床参数之间的相关性，绘制受试者工作特征（ROC）曲线，并比较曲线下面积（AUC）值，以探索其在患者中的早期诊断效用。
结果
我们首次发现CCA中合胞素-1和SLC7A11的胆汁cfDNA水平高于胆结石患者（3.06对1.32，p＜0.001；2.39对1.30，p＜0.001）。合胞素-1和SLC7A11表达之间存在显著相关性（p = 0.025）。此外，合胞素-1或SLC7A11的胆汁cfDNA在胆结石、胆管癌I-II期和胆管癌III-IV期存在差异表达（p＜0.001；p＜0.001）。合胞素-1的胆汁cfDNA的AUC为0.805（p＜0.001，特异性为87.0%），SLC7A11的胆汁cfDNA的AUC为0.755（p＜0.001，特异性为80.4%），合胞素-1/SLC7A11/CA19-9标记物的胆汁cDNA组合提高了CCA患者的诊断效率（AUC：0.927，p＜0.001）。
结论
合胞素-1和SLC7A11的胆汁cfDNA在胆管癌中高表达，可作为早期诊断的新型生物标志物。
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