Widiayani Nuniek, Anshori Muhammad Fuad, Nasaruddin Nasaruddin, Farid Muh, Ridwan Ifayanti, Bahrun Abd Haris, Azrai Muhammad, Nur Amin, Khaerani Purnama Isti, Suwarno Willy Bayuardi, Syahruddin Karlina, Khan Naeem, Alotaibi Majed A, Seleiman Mahmoud F
Department of Agronomy, Hasanuddin University, Makassar, 90245, Indonesia.
Indonesian Cereal Testing Instrument Standard Institute, Maros, South Sulawesi, 90514, Indonesia.
BMC Plant Biol. 2025 Jan 29;25(1):122. doi: 10.1186/s12870-025-06103-x.
The development of transgressive segregant (TS) selection on convergent breeding populations of S4 maize is a concept that is rarely applied. However, the development of TS is necessary to accelerate maize breeding pipelines. Therefore, the objectives of this study were (1) to develop the concept of TS selection and (2) to select S4 TS maize to be developed as hybrid cross parents. The study was carried out using two experiments. The first experiment was designed with an augmented design of 6 replications for control genotypes. This design is just one factor focused on maize genotypes. However, it was divided into two sets: non-replicated of 32 TS lines and replicated of four check hybrid varieties. The second experiment focused on validation using a three-way cross. The experiment used a randomized complete block design with three replications. Based on the resulting study, the combination of ratio analysis, path analysis, best linear unbiased prediction, relative fitness, and selection indices is an objective approach to assessing the genetic potential of the S4 TS. The selection index formed was 0.53 ear weight + 0.24-grain yield percentage + yield. The index selection resulted in 11 S4 TS lines being further evaluated for their hybrid potential, with the TS line CB2.23.1 being the best. However, these TSs are expected to focus on identifying and combining ability through diallel crosses in the future. Furthermore, the three-way cross hybrid lines assessment also revealed SG 3.35.12 × JH37 F1 and CB 2.23.1 × JH37 F1 to be promising hybrid lines.
在S4玉米的聚合育种群体上开展渐渗分离株（TS）选择的研究这一概念很少被应用。然而，TS的开发对于加速玉米育种流程是必要的。因此，本研究的目标是：（1）开发TS选择的概念；（2）选择S4 TS玉米作为杂交亲本进行培育。该研究通过两个实验进行。第一个实验采用增广设计，对照基因型重复6次。此设计仅关注一个因素，即玉米基因型。然而，它分为两组：32个TS系不重复，4个对照杂交品种重复。第二个实验侧重于使用三交进行验证。该实验采用随机完全区组设计，重复3次。基于研究结果，比率分析、通径分析、最佳线性无偏预测、相对适合度和选择指数的组合是评估S4 TS遗传潜力的客观方法。形成的选择指数为0.53穗重 + 0.24籽粒产量百分比 + 产量。指数选择产生了11个S4 TS系，进一步评估其杂交潜力，其中TS系CB2.23.1表现最佳。然而，预计这些TS未来将通过双列杂交专注于鉴定和配合力。此外，三交杂交系评估还表明SG 3.35.12×JH37 F1和CB 2.23.1×JH37 F1是有前景的杂交系。
