Lemberger Nick S, Wallmeier Kristin, Fallnich Carsten
Opt Express. 2025 Jan 13;33(1):62-74. doi: 10.1364/OE.540254.
A compact and easy-to-use high-bandwidth autobalanced detector for microscopy is presented, being able to remove up to 67 dB of correlated noise, thus, allowing for shot-noise limited image acquisition even in the presence of high laser excess noise. Detecting a 20 MHz modulation frequency at half the repetition rate of the driving pulsed laser, the autobalanced detector is able to exploit an extra +3 dB increase in signal-to-noise ratio due to the coherent addition of modulation sidebands in stimulated Raman scattering. Pixel-by-pixel noise canceling and correction of sample transmission losses are possible for pixel scan rates of more than 1.7 Mpx/s, enabling full autobalanced operation at imaging speeds of more than 6.5 frames per second for 512 x 512 px images. It is demonstrated that the autobalanced detector is able to separate an overlaying parasitic loss-induced image from a stimulated Raman scattering microscopy image, providing both the noise and transmission corrected stimulated Raman scattering image as well as an attenuation image simultaneously.
本文介绍了一种用于显微镜的紧凑且易于使用的高带宽自动平衡探测器，它能够去除高达67 dB的相关噪声，因此即使在存在高激光过量噪声的情况下也能实现散粒噪声限制的图像采集。该自动平衡探测器以驱动脉冲激光重复频率的一半检测20 MHz的调制频率，由于受激拉曼散射中调制边带的相干相加，能够使信噪比额外提高3 dB。对于超过170万像素/秒的像素扫描速率，可以逐像素进行噪声消除和样品传输损耗校正，对于512×512像素的图像，能够以每秒超过6.5帧的成像速度实现完全自动平衡操作。结果表明，该自动平衡探测器能够将叠加的寄生损耗诱导图像与受激拉曼散射显微镜图像分离，同时提供噪声和传输校正后的受激拉曼散射图像以及衰减图像。
