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芍药苷B4对改善脑缺血/再灌注损伤的作用。

Effect of anemoside B4 on ameliorating cerebral ischemic/reperfusion injury.

作者信息

Fei Huizhi, Huang Xiaohuan

机构信息

Medical college, Jiujiang University, Jiujiang, Jiangxi Province 332200, China.

Department of Basic Medicine, Chongqing Three Gorges Medical College, Chongqing 404100, China.

出版信息

Iran J Basic Med Sci. 2025;28(1):49-55. doi: 10.22038/ijbms.2024.78569.16991.

DOI:10.22038/ijbms.2024.78569.16991
PMID:39877642
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11771334/
Abstract

OBJECTIVES

Anemoside B4 (AB4) is a multifunctional compound with anti-inflammatory, anti-apoptotic, antioxidant, antiviral, and autophagy-enhancing effects. However, the role of AB4 in cerebral ischemia/reperfusion injury (CIRI) remains obscure. This experiment aims to investigate the pharmacological effects of AB4 in CIRI.

MATERIALS AND METHODS

, eighty male SD rats were randomly divided into five groups: sham, MCAO/R, LD group (2.5 mg/kg), MD group (5 mg/kg), and HD group (10 mg/kg). The rats in sham and MCAO/R groups were given equal volumes of normal saline. , PC12 cells were divided into five groups: normal, OGD/R, OGD/R+AB4 (50 μM), OGD/R+AB4 (100 μM), and OGD/R+AB4 (200 μM). The cells were treated with hypoxia and hypoglycemia for 1.5 hr and reoxygenation for 24 hr.

RESULTS

, TTC and neurological scoring tests indicated that AB4 favors promoting the recovery of the brain. The histopathologic study of the brain tissues revealed that AB4 inhibited the damage of neuron cells. The TUNEL assay found that AB4 could improve cell apoptosis and prevent the brain from injury. , the data showed that AB4 inhibited cell damage and prevented PC12 cells from OGD/R injury, reduced IL-1β content, and increased the IL-10 level. AB4 could inhibit apoptosis of PC12 cells, down-regulate Caspase 12 and BAX expression, and up-regulate Bcl-2 expression.

CONCLUSION

AB4 played a protective role in CIRI and could be a promising active ingredient against ischemia stroke.

摘要

目的

芍药苷B4（AB4）是一种具有抗炎、抗凋亡、抗氧化、抗病毒和增强自噬作用的多功能化合物。然而，AB4在脑缺血/再灌注损伤（CIRI）中的作用仍不清楚。本实验旨在研究AB4在CIRI中的药理作用。

材料与方法

80只雄性SD大鼠随机分为五组：假手术组、大脑中动脉闭塞/再灌注（MCAO/R）组、低剂量组（2.5mg/kg）、中剂量组（5mg/kg）和高剂量组（10mg/kg）。假手术组和MCAO/R组大鼠给予等体积的生理盐水。此外，PC12细胞分为五组：正常组、氧糖剥夺/再灌注（OGD/R）组、OGD/R + AB4（50μM）组、OGD/R + AB4（100μM）组和OGD/R + AB4（200μM）组。细胞经缺氧和低血糖处理1.5小时，再复氧24小时。

结果

TTC和神经功能评分测试表明，AB4有利于促进脑功能恢复。脑组织的组织病理学研究表明，AB4可抑制神经元细胞损伤。TUNEL检测发现，AB4可改善细胞凋亡并预防脑损伤。此外，数据显示，AB4可抑制细胞损伤，预防PC12细胞OGD/R损伤，降低IL-1β含量，并提高IL-10水平。AB4可抑制PC12细胞凋亡，下调Caspase 12和BAX表达，并上调Bcl-2表达。

结论

AB4在CIRI中发挥了保护作用，可能是一种有前途的抗缺血性中风活性成分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/fb6b2e4960c3/IJBMS-28-49-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/b6488001936d/IJBMS-28-49-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/7f4c0c34b71e/IJBMS-28-49-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/178058a9bbc4/IJBMS-28-49-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/5d65a190bb09/IJBMS-28-49-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/568d8fb6bee4/IJBMS-28-49-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/5bcdeb3142f4/IJBMS-28-49-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/fb6b2e4960c3/IJBMS-28-49-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/b6488001936d/IJBMS-28-49-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/7f4c0c34b71e/IJBMS-28-49-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/178058a9bbc4/IJBMS-28-49-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/5d65a190bb09/IJBMS-28-49-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/568d8fb6bee4/IJBMS-28-49-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/5bcdeb3142f4/IJBMS-28-49-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c43/11771334/fb6b2e4960c3/IJBMS-28-49-g007.jpg

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