对招潮蟹（学名：Uca pugilator）中黑色色素分散激素的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay of black pigment dispersing hormone from the fiddler crab, Uca pugilator.
作者信息
Quackenbush L S, Fingerman M
出版信息
Gen Comp Endocrinol. 1985 Mar;57(3):438-44. doi: 10.1016/0016-6480(85)90226-6.
PMID:3988026
Abstract
An enzyme-linked immunosorbent assay was developed for black pigment dispersing hormone (BPDH) obtained from the fiddler crab, Uca pugilator. Immunoreactive BPDH, as high as 8.8 +/- 0.9 ng/50 microliters, was found in the hemolymph of crabs with dispersed black pigment. High potassium saline caused 4.5 +/- 0.4 ng immunoreactive BPDH to be released into the medium from isolated crab eyestalks in 5 min.
摘要
开发了一种酶联免疫吸附测定法，用于检测从招潮蟹（学名：Uca pugilator）中获得的黑色色素分散激素（BPDH）。在黑色色素已分散的螃蟹血淋巴中发现了免疫反应性BPDH，含量高达8.8±0.9纳克/50微升。高钾盐水可使分离出的螃蟹眼柄在5分钟内将4.5±0.4纳克免疫反应性BPDH释放到培养基中。
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