Gião T, Müller M I, Yamada F, Freitas F, Leite L, da Silva R J, de Azevedo R, Abdallah V
Universidade Federal de Santa Catarina (UFSC), Florianópolis, Santa Catarina, Brasil.
Oregon State University (OSU), Department of Microbiology, Corvallis, Oregon, USA.
J Helminthol. 2025 Jan 30;99:e13. doi: 10.1017/S0022149X24000993.
Here, we present a comprehensive morphological and molecular phylogenetic analysis of sp. (Digenea: Clinostomidae) metacercariae parasitizing two freshwater fish species from Southeast Brazil: (piranha) and (tambuatá). The morphological examination revealed distinct characteristics of metacercariae in each host. Using the cytochrome c oxidase I (COI) gene barcode region, we obtained DNA sequences that allowed for accurate phylogenetic placement. Phylogenetic analyses revealed that sp. HM41 (metacercariae), isolated from , exhibited 86% similarity to , while sp. HM125 (metacercariae), from , showed 98.7% similarity to sp. Cr_Ha1. The phylogenetic trees constructed through Bayesian Inference and Maximum Likelihood methods indicated high biodiversity within the genus and strong support for distinct lineages. These findings enhance our understanding of the diversity and ecological distribution of species in South American freshwater environments.
在此，我们对寄生于巴西东南部两种淡水鱼——食人鲳（Pygocentrus nattereri）和坦布阿塔（Mylossoma aureum）体内的曲口吸虫属（复殖目：曲口科）囊蚴进行了全面的形态学和分子系统发育分析。形态学检查揭示了每种宿主体内囊蚴的不同特征。利用细胞色素c氧化酶I（COI）基因条形码区域，我们获得了能够进行准确系统发育定位的DNA序列。系统发育分析表明，从食人鲳分离出的曲口吸虫属HM41囊蚴与曲口吸虫属某物种表现出86%的相似性，而从坦布阿塔分离出的曲口吸虫属HM125囊蚴与曲口吸虫属Cr_Ha1物种表现出98.7%的相似性。通过贝叶斯推断和最大似然法构建的系统发育树表明，曲口吸虫属内具有高度的生物多样性，且对不同谱系有很强的支持。这些发现增进了我们对南美淡水环境中曲口吸虫属物种多样性和生态分布的理解。
