External negative pressure improves lung aeration in near-term rabbit kittens at risk of developing respiratory distress.

INTRODUCTION

As airway liquid is cleared into lung interstitial tissue after birth, the chest wall must expand to accommodate this liquid and the incoming air. We examined the effect of applying external positive and negative pressures to the chest wall on lung aeration in near-term rabbit kittens at risk of developing respiratory distress.

METHODS

Rabbit kittens (30 days; term ∼31 days) were randomised into and groups. Lung liquid was drained in kittens to simulate expected volumes following vaginal delivery. kittens had lung liquid drained before 30 ml/kg was returned to simulate expected volumes after caesarean section. Kittens were delivered, placed in a water-filled plethysmograph and the external pressure was adjusted to -6 (negative), 0 (atmospheric), or +6 (positive) cmHO. Kittens were ventilated with an 8 ml/kg tidal volume and PEEP of 0 cmHO and lungs imaged using phase contrast x-ray imaging.

RESULTS

Compared to external atmospheric pressures, external negative pressures expanded the chest (by 2100 ± 43 vs. 1805 ± 59 mm; kittens;  = 0.028), directed tidal ventilation into lower, larger lung regions and increased functional residual capacity (FRC) levels in both (26.7 ± 2.0 vs. 12.6 ± 2.2 ml/kg;  < 0.001) and (19.6 ± 1.6 vs. 10.0 ± 2.9 ml/kg;  < 0.01) kittens. External positive pressures reduced FRC levels in (6.3 ± 0.8 vs. 12.6 ± 2.2 ml/kg;  < 0.05), but not in kittens, and directed tidal ventilation into upper lung regions.

DISCUSSION

External negative pressures increased lung aeration and resulted in a more evenly distributed tidal ventilation immediately after birth in near-term rabbit kittens, whereas external positive pressures reduced lung aeration and compliance.