Tao Ning, Liu Yuhan, Zhang Ben, Guo Yue, Wang Qingguo, Li Qingqing
College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai'an 271018 Shandong, China.
School of Life Science, Shanxi University, Taiyuan 030006 Shanxi, China.
J Agric Food Chem. 2025 Feb 19;73(7):3897-3907. doi: 10.1021/acs.jafc.4c09064. Epub 2025 Jan 30.
Jasmonic acid (JA) is crucial for plant stress responses, which rely on intercellular jasmonate transport. However, JA transporters have not been fully identified, especially in tomato ( L.). This study reveals that plasma-membrane-localized protein SlABCG9 in tomato regulates fruit resistance to . Transcriptomic analysis indicated that the expression was significantly upregulated after infection. Assays using oocytes, yeast cell sensitivity, and JA-inhibited primary root growth confirmed that SlABCG9 functions as a JA influx transporter. The knockout mutant lines of showed decreased JA contents, suppressed defense gene 's expression, reduced antioxidant enzyme activity, and severe disease symptoms compared to wild-type controls. Our findings provide new knowledge for understanding how the JA transporter and signaling pathway are involved in the biotic stress responses and improve the resistant ability against pathogen infections.
茉莉酸（JA）对植物应激反应至关重要，植物应激反应依赖于细胞间茉莉酸运输。然而，茉莉酸转运蛋白尚未完全鉴定出来，尤其是在番茄中。本研究表明，番茄中定位于质膜的蛋白SlABCG9调节果实对（此处原文缺失相关病原体信息）的抗性。转录组分析表明，（此处原文缺失相关病原体信息）感染后，（此处原文缺失相关基因信息）的表达显著上调。使用卵母细胞、酵母细胞敏感性和茉莉酸抑制初生根生长的实验证实，SlABCG9作为一种茉莉酸内流转运蛋白发挥作用。与野生型对照相比，（此处原文缺失相关基因信息）的敲除突变株系显示茉莉酸含量降低、防御基因（此处原文缺失相关基因信息）的表达受到抑制、抗氧化酶活性降低以及严重的病害症状。我们的研究结果为理解茉莉酸转运蛋白和信号通路如何参与生物应激反应以及提高对病原体感染的抗性能力提供了新知识。
