Castell M, Castellote C, Barbera G
Immunobiology. 1985 Feb;169(1):30-6. doi: 10.1016/S0171-2985(85)80051-6.
We have studied two techniques and developed another to detect blocking antibodies in allergic asthmatic children who have been exposed to seven months of hyposensitization to house dust. These techniques were: RAST Neutralization test (RAST-N), RAST Interference test (RAST-I) and RAST Previous Adsorption test (RAST-PA). We have also determined the specific IgE values, by radioallergosorbent test (RAST), before and after the treatment and have not found a significant decrease in these levels. The percentages of positive development of blocking antibodies obtained were: 28% by RAST-N, 80% by RAST-I and 28% by RAST-PA. Thus, RAST Interference test seems to be more sensitive concerning the qualitative determination of blocking antibodies.
我们研究了两种技术，并开发了另一种技术来检测接触屋尘七个月进行低敏治疗的过敏性哮喘儿童体内的封闭抗体。这些技术分别是：放射性变应原吸附试验中和试验（RAST-N）、放射性变应原吸附试验干扰试验（RAST-I）和放射性变应原吸附试验预先吸附试验（RAST-PA）。我们还通过放射性变应原吸附试验（RAST）测定了治疗前后的特异性IgE值，未发现这些水平有显著下降。获得的封闭抗体阳性发展百分比分别为：RAST-N为28%，RAST-I为80%，RAST-PA为28%。因此，放射性变应原吸附试验干扰试验在封闭抗体的定性测定方面似乎更敏感。
