Wide L
Int Arch Allergy Appl Immunol. 1976;52(1-4):219-26. doi: 10.1159/000231685.
The RAST, originally developed for the assay of reaginic IgE antibodies, was applied for the detection of blocking antibodies. Sera taken ante- or post-hyposensitization were incubated with solid phase coupled allergen which neutralized reaginic IgE antibodies in the serum. The remaining activity of the reaginic IgE antibodies was measured by the RAST. When blocking antibodies were present in the serum they competed with the reaginic IgE antibodies and the amount of reaginic antibodies that was neutralized by the solid phase coupled allergen was decreased. The blocking antibodies were found in the IgG fraction of the serum taken post-hyposensitization. Blocking antibodies to various allergens were detected in 25 out of 26 tests on sera taken post-hyposensitization. The results were compared with those of Prusnitz-Küstner neutralization tests and/or patient self-neutralization tests. There was no correlation between semi-quantitative results of these in vivo tests and those of the RAST neutralization tests. However, cases with subjectively a strong improvement had a significantly higher mean value for RAST neutralization index than those with subjectively a weak improvement. The method is suggested as a supplement to clinical tests for evaluation of hyposensitization treatments.
放射变应原吸附试验（RAST）最初是为检测反应素性IgE抗体而开发的，现用于检测封闭抗体。将脱敏前或脱敏后采集的血清与固相偶联变应原一起孵育，该变应原可中和血清中的反应素性IgE抗体。通过RAST测定反应素性IgE抗体的剩余活性。当血清中存在封闭抗体时，它们会与反应素性IgE抗体竞争，固相偶联变应原中和的反应素性抗体量会减少。在脱敏后采集的血清IgG组分中发现了封闭抗体。在对脱敏后采集的血清进行的26次检测中，有25次检测到了针对各种变应原的封闭抗体。将结果与普-屈二氏中和试验和/或患者自身中和试验的结果进行了比较。这些体内试验的半定量结果与RAST中和试验的结果之间没有相关性。然而，主观上有明显改善的病例，其RAST中和指数的平均值显著高于主观上改善不明显的病例。该方法被建议作为评估脱敏治疗的临床试验的补充。
