Liu Qiao, Peng Junzhi, Tao Ziling, Zhang Jiluan, Wu Weifan, Tan Zhiwu, Zhou Tao, Cao Xiaoying, Jiang Jihong
Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, 221116, China.
World J Microbiol Biotechnol. 2025 Jan 30;41(2):56. doi: 10.1007/s11274-025-04274-1.
Inonotus obliquus (Chaga mushroom) is a large medicinal and edible fungus that contains a wealth of bioactive terpenoids. However, the detection of certain low-abundance sesquiterpenoids remains a challenge due to limitations in extraction and analytical techniques. Furthermore, the synthase genes responsible for the biosynthesis of the identified terpenoids have not yet been clearly elucidated. To address this, our study combined transcriptome mining with yeast heterologous expression to investigate the synthase genes involved in sesquiterpenoid production in I. obliquus. We successfully identified eight sesquiterpene synthase genes and one farnesyltransferase. Among these, only cis-β-farnesene, synthesized by IoTPS2, had been previously detected before in the sclerotium of I. obliquus, while the other nine sesquiterpenoids-including neoisolongifolene-8-ol, β-longipinene, vetiselinenol, isolongifolene, 7,8-dehydro-8a-hydroxy-, 4a,8b,10b,11a-tetramethylbicyclo[6.3.0]undec-1-en-5-one, 6,11-oxido-acor-4-ene, β-maaliene, neointermedeol, and longifolenaldehyde-were discovered for the first time. This research provides a critical scientific foundation for expanding the known repertoire of sesquiterpenoids and their corresponding synthase genes in I. obliquus.
桦褐孔菌是一种大型药食两用真菌，含有丰富的生物活性萜类化合物。然而，由于提取和分析技术的限制，某些低丰度倍半萜类化合物的检测仍然是一个挑战。此外，负责已鉴定萜类化合物生物合成的合酶基因尚未明确阐明。为了解决这个问题，我们的研究将转录组挖掘与酵母异源表达相结合，以研究桦褐孔菌中参与倍半萜类化合物产生的合酶基因。我们成功鉴定出8个倍半萜合酶基因和1个法尼基转移酶。其中，只有由IoTPS2合成的顺式-β-法尼烯之前在桦褐孔菌的菌核中被检测到，而其他9种倍半萜类化合物——包括新异长叶烯-8-醇、β-长叶蒎烯、维替塞林醇、异长叶烯、7,8-脱氢-8a-羟基-、4a,8b,10b,11a-四甲基双环[6.3.0]十一碳-1-烯-5-酮、6,11-环氧-菖蒲-4-烯、β-马勒烯、新间苯三酚和长叶蒎醛——是首次发现。这项研究为扩大桦褐孔菌中已知的倍半萜类化合物及其相应合酶基因库提供了关键的科学基础。
