Lin Jiahao, Zhang Jiannan, Zhu Xianglin, Xia Xuhan, Zhang Yong, Zeng Qingdong, Xu Yuanhong, Deng Ruijie, Li Jinghong
Institute of Biomedical Engineering, College of Life Sciences, Qingdao University, Qingdao 266071, China.
Beijing Life Science Academy, Beijing 102206, China.
Chem Commun (Camb). 2025 Feb 18;61(16):3335-3338. doi: 10.1039/d4cc06481j.
Detection of low-abundance mutations for the early discovery of fungicide-resistant fungal pathogens is highly demanded, but remains challenging. Herein, we developed a dual-recognition strategy, termed PARPA, involving s Argonaute (pfAgo)-mediated elimination of wild-type fungal genes and CRISPR/Cas12a-based amplicon recognition. This assay can detect fungicide-resistant at relative abundances as low as 0.05% and has potential for achieving early screening of fungicide-resistant fungal pathogens.
对于早期发现抗真菌剂的真菌病原体而言，检测低丰度突变的需求迫切，但仍具有挑战性。在此，我们开发了一种双识别策略，称为PARPA，它涉及嗜热栖热菌Argonaute（pfAgo）介导的野生型真菌基因消除和基于CRISPR/Cas12a的扩增子识别。该检测方法能够检测低至0.05%相对丰度的抗真菌剂，并具有实现抗真菌剂抗性真菌病原体早期筛查的潜力。
