Fu Ruijie, Hou Jinjie, Wang Zexiang, Zhu Chenggong, Xianyu Yunlei
Department of Clinical Laboratory of Sir Run Run Shaw Hospital, College of Biosystems Engineering and Food Science, Zhejiang University School of Medicine, Hangzhou 310058, People's Republic of China.
Key Laboratory of Precision Medicine in Diagnosis and Monitoring Research of Zhejiang Province, Sir Run Run Shaw Hospital, Hangzhou 310016, People's Republic of China.
ACS Nano. 2025 Feb 4;19(4):4983-4992. doi: 10.1021/acsnano.4c17560. Epub 2025 Jan 24.
The escalating growth in computing power and the advent of quantum computing present a critical threat to the security of modern cryptography. Two-factor authentication strategies can effectively resist brute-force attacks to improve the security of access control. Herein, we proposed a two-factor and two-authentication entity strategy based on the trans-cleavage activity of CRISPR-Cas and the "dual-step" sequence-specific cleavage of Argonaute. In this strategy, the output of authentication entity 1 acted as a component to operate authentication entity 2, thus enabling a role-based molecular model that implemented access control for the three roles. To further enhance information security, we designed knowledge suppression factors to constitute the command library and possession suppression factors to resist brute-force attacks. This study will promote the development of advanced molecular access control and its applications in biomedical diagnostics and data security.
计算能力的不断提升以及量子计算的出现对现代密码学的安全性构成了重大威胁。双因素认证策略可以有效抵御暴力攻击，从而提高访问控制的安全性。在此，我们基于CRISPR-Cas的反式切割活性和Argonaute的“两步”序列特异性切割，提出了一种双因素双认证实体策略。在该策略中，认证实体1的输出作为操作认证实体2的一个组件，从而实现了一种基于角色的分子模型，该模型对三个角色实施访问控制。为了进一步增强信息安全，我们设计了知识抑制因子来构成命令库，并设计了占有抑制因子来抵御暴力攻击。本研究将推动先进分子访问控制的发展及其在生物医学诊断和数据安全中的应用。
