Hasan Anamul, Hossain Md Mobarok, Zamil Md Fahad, Trina Afrida Tabassum, Hossain Mohammad Sharif, Kumkum Asifa, Afreen Sajia, Ahmed Dilruba, Rahman Mustafizur, Alam Mohammad Shafiul
Infectious Diseases Division, International Centre for Diarrheal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh.
Clinical and Diagnostic Services, International Centre for Diarrheal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh.
PLoS Negl Trop Dis. 2025 Jan 30;19(1):e0012866. doi: 10.1371/journal.pntd.0012866. eCollection 2025 Jan.
During the 2023-dengue outbreak in Bangladesh, a diagnostic evaluation study was conducted to investigate concurrent Zika virus (ZIKV) and dengue virus (DENV) transmission in Dhaka in 2023.
The study explored to simultaneously detect the presence of ZIKV, DENV, and/or CHIKV while considering relevant clinical and epidemiological risk factors, using a real-time multiplex RT-PCR system. Following this, it was planned to sequence the selected samples to identify genetic variations of the ZIKV infections within the population.
This study was designed as a diagnostic evaluation, where participants meeting the inclusion criteria were prospectively recruited with written informed consent. A total of 399 febrile individuals were screened, with 185 meeting the inclusion criteria of having a fever onset within 2-5 days, along with one of the following clinical features, e.g. headache, myalgia, arthralgia or bone pain, rash, nausea, vomiting, or diarrhea and 152 undergoing real-time RT-PCR testing.
Five ZIKV-positive cases were identified, including one DENV-ZIKV co-infection. Phylogenetic analysis revealed the ZIKV strains were part of the Asian lineage, closely related to Cambodian and Chinese strains from 2019. All ZIKV-positive cases were male, residing within a one-kilometer radius, with no prior travel history, suggesting community-level transmission.
This study marks the first identification of ZIKV in Dhaka city and the first report of ZIKV-DENV co-infection in Bangladesh that highlights the diagnostic challenges posed by the symptomatic similarities between ZIKV and other arboviruses and underscores the need for enhanced surveillance and public health interventions to mitigate the spread and impact of ZIKV in dengue-endemic regions.
在2023年孟加拉国登革热疫情期间，开展了一项诊断评估研究，以调查2023年达卡市寨卡病毒（ZIKV）和登革热病毒（DENV）的同时传播情况。
本研究旨在使用实时多重逆转录聚合酶链反应（RT-PCR）系统，在考虑相关临床和流行病学风险因素的同时，探索同时检测寨卡病毒、登革热病毒和/或基孔肯雅病毒（CHIKV）的存在情况。在此之后，计划对选定样本进行测序，以识别该人群中寨卡病毒感染的基因变异。
本研究设计为诊断评估，符合纳入标准的参与者在获得书面知情同意后被前瞻性招募。共筛查了399名发热个体，其中185名符合纳入标准，即发热发病在2至5天内，伴有以下临床特征之一，如头痛、肌痛、关节痛或骨痛、皮疹、恶心、呕吐或腹泻，152名接受了实时RT-PCR检测。
鉴定出5例寨卡病毒阳性病例，包括1例登革热病毒-寨卡病毒合并感染。系统发育分析显示，寨卡病毒株属于亚洲谱系，与2019年柬埔寨和中国的毒株密切相关。所有寨卡病毒阳性病例均为男性，居住在半径1公里范围内，无前科旅行史，提示存在社区层面传播。
本研究标志着达卡市首次发现寨卡病毒，也是孟加拉国寨卡病毒-登革热病毒合并感染的首次报告，突出了寨卡病毒与其他虫媒病毒症状相似性带来的诊断挑战，并强调需要加强监测和公共卫生干预措施，以减轻寨卡病毒在登革热流行地区的传播和影响。
