Thanh Phạm Huyền Thị, Kuroda Shohei, Khairina Yeni, Morikawa Masaaki
Division of Biosphere Science, Graduate School of Environmental Science, Hokkaido University, Kita-10, Nishi-5, Kita-ku, Sapporo 060-0810, Japan.
Division of Biosphere Science, Graduate School of Environmental Science, Hokkaido University, Kita-10, Nishi-5, Kita-ku, Sapporo 060-0810, Japan; Duckweed Holobiont Resource & Research Center (DHbRC), Kasetsart University, Thailand.
Bioresour Technol. 2025 Apr;421:132110. doi: 10.1016/j.biortech.2025.132110. Epub 2025 Jan 28.
Duckweed has been highlighted as an appropriate biomass for low-carbon industries because of its significantly high production rate and multiple resource value. However, the outbreak of microalgae is a practical issue that decreases duckweed production yield. This study demonstrated that the growth of the duckweed Lemna aequinoctialis from factory wastewater was enhanced by colonization with indigenous plant growth-promoting bacteria (PGPB), whereas the growth of a duckweed competitor microalga, Coelastrella sp. KC10, from the same wastewater was reduced by indigenous microalgal growth-inhibiting bacteria (MGIB). Finally, a quadruple co-culture of a synthetic duckweed holobiont, L. aequinoctialis colonized by both KLaR20 (PGPB) and KLaR16 (MGIB), and Coelastrella sp. KC10 successfully recovered the duckweed production level by 117.5% in frond number and 84.5% in dry weight compared to those in the absence of microalgae. This case study demonstrates for the first time that duckweed holobionts can be reconstructed and enforced to antagonize growth competitor microalgae.
浮萍因其极高的生产率和多种资源价值，已成为低碳产业的合适生物质。然而，微藻爆发是一个降低浮萍产量的实际问题。本研究表明，本地植物促生细菌（PGPB）定殖可促进源自工厂废水的浮萍品系小浮萍（Lemna aequinoctialis）的生长，而源自同一废水的浮萍竞争微藻绿球藻（Coelastrella sp. KC10）的生长则受到本地微藻生长抑制细菌（MGIB）的抑制。最后，由KLaR20（PGPB）和KLaR16（MGIB）定殖的合成浮萍共生体小浮萍与绿球藻（Coelastrella sp. KC10）的四重共培养，与无微藻时相比，成功使浮萍产量在叶状体数量上提高了117.5%，干重提高了84.5%。本案例研究首次证明，浮萍共生体可以重建并强化以对抗生长竞争微藻。
