Fucoidan and chitosan electrostatically coated nanoliposomes enhance physicochemical stability and bioavailability of rutin.

Rutin, a promising bioactive hydrophobic compound, suffers from poor physicochemical stability, resulting in low bioavailability. Herein, we used positively charged chitosan and negatively charged fucoidan as biopolymers coating rutin-nanoliposome (RNL) via electrostatic layer-by-layer self-assembly approach to prepare fucoidan/chitosan-coated rutin-nanoliposome (FC-RNL). The FC-RNL exhibited the encapsulation efficiency of 77.01% for rutin, with the particle size of 346 nm and a zeta potential of -33.5 mV under the optimized conditions (lecithin to rutin ratio of 10, 0.05 wt% fucoidan and 0.20 wt% chitosan). The results of Fourier transform infrared, X-ray diffraction, and transmission electron microscopy suggested that fucoidan/chitosan-coated nanoliposome could effectively load rutin. The coating of fucoidan and chitosan not only improved the retention rate of rutin (> 85 %) under thermal, oxidative and UV-light conditions, but also showed excellent stability over a wide pH range (pH 3.0-11.0) and high ionic strength (400 mM NaCl). In addition, FC-RNL was more stable than C-RNL and RNL at 4 °C for 5-week storage. In vitro simulated digestion indicated that FC-RNL significantly controlled the rutin release, and preserved 6.86 % and 50.47 % of rutin at the end of simulated gastric and intestinal digestion, respectively. Furthermore, FC-RNL exhibited satisfactory biocompatibility, and cellular uptake studies demonstrated that FC-RNL displayed the highest Rh123 uptake efficiency reaching approximately 189 %. This study provides an effective fucoidan/chitosan-coated nanoliposome carrier for the delivery of hydrophobic bioactive compounds within the functional food industry.