Khan Farhin, Mondal Bidya, Bairagi Baishakhi, Mandal Sukanta, Mandal Dipankar, Nath Debjani
Department of Zoology, University of Kalyani, Kalyani, Nadia, West Bengal 741235, India.
Quantum Materials and Devices Unit, Institute of Nano Science and Technology, Knowledge City, Sector-81, Mohali 140306, India.
Int J Biol Macromol. 2025 Apr;301:140416. doi: 10.1016/j.ijbiomac.2025.140416. Epub 2025 Jan 28.
The pathophysiological relationship between wound healing impairment and diabetes is an intricate process. Burn injury among diabetes patients leads to neurological, vascular, and immunological abnormalities along with impaired activities of cell proliferation, collagen production, growth factors, and cytokine activities with huge bacterial infestation. In our study, we aimed to achieve a burn wound dressing material with the help of electrospun Chitosan/Polyethylene oxide/Rosmarinic acid (CS/PEO/RA) nanofibers. Chitosan is known for its biocompatibility and anti-bacterial properties; however, the electrospinning of CS requires a co-polymer such as PEO, a synthetic biodegradable polymer. With the addition of a low concentration of RA, known for its antibacterial, antioxidative nature, we enhanced the antibacterial efficacy of the electrospun nanofiber. Electrospinning CS/PEO/RA, we were able to develop a non-toxic scaffold with fibers having an average diameter of 127.035 nm, mimicking the extracellular matrix and exhibiting sustained drug release. Excellent antimicrobial activity was observed against the identified bacterial species. It showed increased wound contraction and reduced scar formation in the diabetic mice model along with rapid repair of the damaged epithelial barrier. It enhanced the production of collagen, elastin, and α-smooth muscle actin (α-SMA). Thus, it justifies itself as a diabetic burn wound dressing at low drug concentration.
伤口愈合受损与糖尿病之间的病理生理关系是一个复杂的过程。糖尿病患者的烧伤会导致神经、血管和免疫异常，同时细胞增殖、胶原蛋白生成、生长因子和细胞因子活性受损，并伴有大量细菌感染。在我们的研究中，我们旨在借助静电纺丝的壳聚糖/聚环氧乙烷/迷迭香酸（CS/PEO/RA）纳米纤维制备一种烧伤创面敷料材料。壳聚糖以其生物相容性和抗菌特性而闻名；然而，CS的静电纺丝需要一种共聚物，如PEO，一种合成的可生物降解聚合物。添加低浓度的RA，其以抗菌、抗氧化性质而闻名，我们增强了静电纺纳米纤维的抗菌效果。通过静电纺丝CS/PEO/RA，我们能够开发出一种无毒支架，其纤维平均直径为127.035纳米，模仿细胞外基质并表现出持续的药物释放。对鉴定出的细菌物种观察到了优异的抗菌活性。在糖尿病小鼠模型中，它显示出伤口收缩增加、瘢痕形成减少以及受损上皮屏障的快速修复。它增强了胶原蛋白、弹性蛋白和α-平滑肌肌动蛋白（α-SMA）的产生。因此，它证明自己是一种低药物浓度的糖尿病烧伤创面敷料。
