Liu Chengzhi, Zhong Yawen, Xiang Yifei, Qin Lanqian, Li Jiada, Huang Luyuan, Ning Junkai, He Weiwei, Wu Chunxuan, Cheng Qiuqi, Yi Dandan, Liu Niu, Xu Chuang, Liang Zhengmin, He Jiakang
Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi 530004, China.
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Int J Biol Macromol. 2025 Apr;301:140431. doi: 10.1016/j.ijbiomac.2025.140431. Epub 2025 Jan 28.
The application of hydrogels to drug delivery limited by the difficulty of encapsulating hydrophobic drugs; therefore, the development of novel composite hydrogels for the delivery of hydrophobic drugs is urgently needed. In this study, terbinafine hydrochloride/hydroxypropyl-β-cyclodextrin inclusion complexes (TFH/HP-β-CD ICs) were added to a Schiff base hydrogel matrix containing octenyl succinic anhydride-modified chitosan (OSA-CS) and sodium alginate (OIA) to prepare a TFH composite hydrogel (TFH GEL). The results revealed that the solubility of TFH in water within TFH/HP-β-CD IC reached 32.13 mg/mL. The TFH GEL successfully encapsulated the IC without any drug leakage and exhibited excellent acid pH responsiveness. Moreover, the hydrogels were mechanically stable, self-healing, and injectable. Haemocompatibility and cytotoxicity tests confirmed the excellent biocompatibility of the TFH GEL. Importantly, TFH GEL effectively inhibited Microsporum canis growth in vitro and in vivo. In summary, a novel composite hydrogel was developed by combining a modified natural polymer hydrogel with a complexing agent to deliver the hydrophobic antifungal drug TFH, this study provides a new strategy for treating fungal skin infections.
水凝胶在药物递送中的应用受到包封疏水药物困难的限制；因此，迫切需要开发用于递送疏水药物的新型复合水凝胶。在本研究中，将盐酸特比萘芬/羟丙基-β-环糊精包合物（TFH/HP-β-CD ICs）添加到含有辛烯基琥珀酸酐改性壳聚糖（OSA-CS）和海藻酸钠（OIA）的席夫碱水凝胶基质中，制备了TFH复合水凝胶（TFH GEL）。结果表明，TFH在TFH/HP-β-CD IC中的水中溶解度达到32.13 mg/mL。TFH GEL成功包封了IC，没有任何药物泄漏，并表现出优异的酸pH响应性。此外，水凝胶具有机械稳定性、自愈合性和可注射性。血液相容性和细胞毒性测试证实了TFH GEL具有优异的生物相容性。重要的是，TFH GEL在体外和体内均有效抑制犬小孢子菌生长。总之，通过将改性天然聚合物水凝胶与络合剂相结合，开发了一种新型复合水凝胶来递送疏水抗真菌药物TFH，本研究为治疗真菌性皮肤感染提供了一种新策略。
