Zhang Hua, Feng Zhao-Yuan, Li Zhen-Zhou, Wang Yi-Sen, Mu Zhe-Xuan, Lv Xiao-Hong, Wang Zhen-Yu, Fan Zi-Luan
Innovation Research Center for Special Food-Medicine and Biochemical Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang, China.
College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang, China.
Int J Biol Macromol. 2025 Apr;303:140414. doi: 10.1016/j.ijbiomac.2025.140414. Epub 2025 Jan 28.
In this study, a polysaccharide component was extracted from Pinus pumila using biomimetic-microwave assisted alkali tandem extraction and then purified by DEAE-52 and Sephadex G-200. A new purified polysaccharide (PSP c-a) was determined through in vitro tracking. The structural features were characterized using a HPGP, FTIR, IC and NMR spectroscopy. The analysis showed that there may be →3, 6)-α-Galp (1 → 3, 4)-α-Glcp (1 → 5)-α-Manp (1→ in the backbone of PSP c-a, →3, 4)-α-Glcp (1→ and →3, 4)-α-Glcp (1 → α-Arap (1→ in the branch of PSP c-a. To improve the effects of PSP c-a on osteoblast MC3T3-E1, PSP c-a was biologically and chemically modified by α-glucosidase together with Sr. Finally, a new thermostable complex Sr-PSP c-a-1 was synthesized. The effects of PSP c-a and Sr-PSP c-a-1 on osteoblasts MC3T3-E1 were compared. It was found that both were found to have a positive effect on MC3T3-E1, and Sr-PSP c-a-1 was more effective than PSP c-a. Therefore, Sr-PSP c-a-1 should be a potential candidate for promoting skeletal tissue regeneration.
在本研究中，采用仿生-微波辅助碱串联萃取法从偃松中提取多糖成分，然后通过DEAE-52和Sephadex G-200进行纯化。通过体外追踪确定了一种新的纯化多糖（PSP c-a）。利用高效凝胶渗透色谱（HPGP）、傅里叶变换红外光谱（FTIR）、离子色谱（IC）和核磁共振光谱（NMR）对其结构特征进行了表征。分析表明，PSP c-a的主链中可能存在→3, 6)-α-Galp (1→、3, 4)-α-Glcp (1→、5)-α-Manp (1→，PSP c-a的支链中存在→3, 4)-α-Glcp (1→和→3, 4)-α-Glcp (1→α-Arap (1→。为提高PSP c-a对成骨细胞MC3T3-E1的作用，将PSP c-a与α-葡萄糖苷酶和锶进行生物和化学修饰。最后，合成了一种新型热稳定复合物Sr-PSP c-a-1。比较了PSP c-a和Sr-PSP c-a-1对成骨细胞MC3T3-E1的影响。发现两者对MC3T3-E1均有积极作用，且Sr-PSP c-a-1比PSP c-a更有效。因此，Sr-PSP c-a-1应是促进骨骼组织再生的潜在候选物。
