Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150090, Heilongjiang, China.
Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150090, Heilongjiang, China; Tianjin Institute of Quality Standard and Testing Technology for Agro-product, Tianjin Academy of Agricultural Sciences, Tianjin, 300380, China.
Carbohydr Polym. 2021 Jan 1;251:117078. doi: 10.1016/j.carbpol.2020.117078. Epub 2020 Sep 11.
The polysaccharides (PKP-E) extracted from the pinecones of Pinus koraiensis were studied, which was fractionated using DEAE-52 cellulose and Sephadex G-100. Four novel polysaccharide fractions were obtained, which were PKP-E-1-1, -1-2, -2-1, and -2-2, respectively. The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy. The results showed the 4 purified fractions were non-triple helical structured heteropolysaccharides and composed of l-rhamnose, l-arabinose, d-mannose, d-glucose, and d-galactose. The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin. Moreover, the antioxidant activities of the polysaccharides were measured by scavenging radical capacity tests. The PKP-E-2-1 was the most stable and active fraction, and the respective IC for the hydroxyl and ABTS radicals were 3.0 and 23.6 mg/mL.
从红松的松果中提取的多糖（PKP-E），经 DEAE-52 纤维素和 Sephadex G-100 进行了分级分离。得到了四个新型多糖级分，分别为 PKP-E-1-1、-1-2、-2-1 和-2-2。采用 HPGPC、单糖组成分析、刚果红试验、高碘酸氧化、Smith 降解、FTIR 和 NMR 光谱对其结构特征进行了表征。结果表明，4 种纯化级分均为非三螺旋结构的杂多糖，由 l-鼠李糖、l-阿拉伯糖、d-甘露糖、d-葡萄糖和 d-半乳糖组成。这些级分主要通过 1→6 或 1→糖苷键连接，4 个级分的骨架可能由→2,6)-β-d-Man-(1→和α-d-GalpA-(1→组成，类似于果胶。此外，通过清除自由基能力试验测定了多糖的抗氧化活性。PKP-E-2-1 是最稳定和最活跃的级分，其对羟基和 ABTS 自由基的 IC 分别为 3.0 和 23.6mg/mL。
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