用深共熔溶剂提取花青素并通过高效液相色谱法进行化学评估。
Extraction of Anthocyanins with Deep Eutectic Solvents and Chemical Evaluation by High-Performance Liquid Chromatography.
作者信息
Dai Yuntao, Verpoorte Robert, Choi Young Hae
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Natural Products Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands.
出版信息
Methods Mol Biol. 2025;2895:63-72. doi: 10.1007/978-1-0716-4350-1_5.
Deep eutectic solvents (DES) and ionic liquids (ILs), specifically natural deep eutectic solvents (NADES), allow for the extraction of natural products using environmentally friendly solvents instead of organic solvents. Here we describe the extraction of anthocyanins from a medicinal plant using NADES prepared either by evaporating method or heating-and-stirring method with the help of ultrasound-assisted extraction (UAE). The NADES extract can be qualified by the high-performance liquid chromatography (HPLC) method, which can separate the component of NADES with target compounds from medicinal plant.
低共熔溶剂（DES）和离子液体（ILs），特别是天然低共熔溶剂（NADES），允许使用环境友好型溶剂而非有机溶剂来提取天然产物。在此，我们描述了借助超声辅助提取（UAE），使用通过蒸发法或加热搅拌法制备的NADES从一种药用植物中提取花青素的过程。NADES提取物可用高效液相色谱（HPLC）法进行鉴定，该方法能够将NADES的成分与药用植物中的目标化合物分离。
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