COX2/mPGES1/PGE2在小鼠和人类子宫内膜基质蜕膜化中的作用
Participation of COX2/mPGES1/PGE2 in mouse and human endometrial stromal decidualization.
作者信息
Wang Peng-Chao, Liu Jie, Liu Yue-Fang, Wu Yang, Xue Lin-Li, Yang Zhen-Shan
机构信息
College of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Jinzhong, China.
Dakewe Biotech Co., Ltd., Guangzhou, 510642, China.
出版信息
BMC Vet Res. 2025 Jan 30;21(1):43. doi: 10.1186/s12917-025-04505-5.
BACKGROUND
Prostaglandin E2 (PGE2) is vital for embryo implantation and decidualization. Whether COX2/mPGES1/PGE2 pathway is essential for mouse and human decidualization remains unclear.
RESULTS
This study showed that mPGES1 was highly expressed in the mouse uterus's subluminal stromal cells at the implantation site. COX2-specific inhibitor Valdecoxib and mPGES1 selective inhibitor MK886 were used to analyze the roles of mPGES1 and COX2 during mouse and human decidualization. During mouse in vitro decidualization, decidua/trophoblast prolactin-related protein (Dtprp) expression was significantly suppressed by Valdecoxib and MK886. Under human in vitro decidualization, mPGES1 significantly increases, while both cPGES and mPGES2 remain unchanged. PGE2-mediated upregulation of insulin growth factor binding protein 1 (IGFBP1) was significantly inhibited by Valdecoxib and MK886.
CONCLUSIONS
Our findings suggest the involvement of COX2/mPGES1/PGE2 pathway in both mouse and human decidualization.
背景
前列腺素E2（PGE2）对胚胎着床和蜕膜化至关重要。COX2/mPGES1/PGE2通路对小鼠和人类的蜕膜化是否必不可少仍不清楚。
结果
本研究表明，mPGES1在小鼠子宫着床部位的腔下基质细胞中高表达。使用COX2特异性抑制剂伐地考昔和mPGES1选择性抑制剂MK886来分析mPGES1和COX2在小鼠和人类蜕膜化过程中的作用。在小鼠体外蜕膜化过程中，伐地考昔和MK886显著抑制蜕膜/滋养层催乳素相关蛋白（Dtprp）的表达。在人类体外蜕膜化过程中，mPGES1显著增加，而cPGES和mPGES2均保持不变。伐地考昔和MK886显著抑制PGE2介导的胰岛素生长因子结合蛋白1（IGFBP1）上调。
结论
我们的研究结果表明COX2/mPGES1/PGE2通路参与了小鼠和人类的蜕膜化过程。
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