Wu A, Lin P S
Int J Radiat Oncol Biol Phys. 1985 May;11(5):983-6. doi: 10.1016/0360-3016(85)90121-x.
Exponentially growing Chinese hamster V79 cells were heated from 37 degrees C up to 42 degrees C or 43 degrees C at four different heating rates: immediate exposure, 1/2 hour, 1 hour, and 2 hours. The D0's of the heat survival curves for both 42 degrees C and 43 degrees C were determined for the different heating rates. The results show that the decrease in toxicity to cells at 43 degrees C resulting from a slower heating rate appears to be less pronounced than that at 42 degrees C. However, the thermotolerance ratio is found to depend on the specific heating time (the reciprocal of the heating rate), in a linear fashion and it does not seem to depend on the peak temperature. We propose that the difference in the heat exchange on cell membranes at different rates of heating may contribute to the causes for the difference in cell sensitivity to hyperthermia.
将指数生长的中国仓鼠V79细胞以四种不同的加热速率从37℃加热到42℃或43℃：立即暴露、半小时、1小时和2小时。针对不同的加热速率，测定了42℃和43℃时热存活曲线的D0值。结果表明，加热速率较慢导致的43℃时细胞毒性降低似乎不如42℃时明显。然而，发现热耐受比以线性方式取决于特定的加热时间（加热速率的倒数），并且似乎不取决于峰值温度。我们提出，不同加热速率下细胞膜上热交换的差异可能是导致细胞对热疗敏感性差异的原因。
