Jiang Y S, Law P
Proc Natl Sci Counc Repub China B. 1985 Oct;9(4):269-74.
The survival rate of V79 Chinese hamster lung fibroblasts treated with heat and freezing was investigated. The hyperthermic behavior of these cells was similar to other reports; Thermotolerance was observed below 43 degrees C during continuous heating. There was no difference in survival rate whether the cells were heated while attached to tissue culture flasks or in suspension, although the pH of the medium during the latter situation could be around 8.2 during hyperthermic treatment. If the cells were frozen and thawed before heating, the pattern of survival did not change significantly from that of heating alone, and thermotolerance was still observed below 43 degrees C. The Arrhinius plot of heat sensitivities between 41 and 45 degrees C demonstrated a straight line, giving an activation energy of 171 kcal/mole. A break on the plot around 42.5 degrees C could not be located, probably due to the lack of experimental points. If the cells were frozen and thawed immediately after heating, the survival rate was lower than that expected from considering hyperthermia and freezing as two independent processes. Possible explanations are provided in the result and discussion section.
研究了经加热和冷冻处理的V79中国仓鼠肺成纤维细胞的存活率。这些细胞的热行为与其他报道相似；在持续加热过程中，43℃以下观察到热耐受性。细胞附着于组织培养瓶中加热或悬浮加热时，存活率没有差异，尽管在后一种情况下，热疗期间培养基的pH值可能约为8.2。如果细胞在加热前冷冻和解冻，存活模式与单独加热相比没有显著变化，并且在43℃以下仍观察到热耐受性。41至45℃之间热敏感性的阿累尼乌斯图呈直线，活化能为171千卡/摩尔。可能由于缺乏实验点，在约42.5℃处未找到该图上的断点。如果细胞在加热后立即冷冻和解冻，存活率低于将热疗和冷冻视为两个独立过程时预期的存活率。结果与讨论部分提供了可能的解释。
