Park Sohyun, Kim Kihyun, Go Anna, Lee Min-Ho, Chen Lingxin, Choo Jaebum
Department of Chemistry, Chung-Ang University, Seoul 06974, South Korea.
School of Integrative Engineering, Chung-Ang University, Seoul 06974, South Korea.
ACS Sens. 2025 Feb 28;10(2):1217-1227. doi: 10.1021/acssensors.4c03118. Epub 2025 Jan 30.
Bacterial infections, such as sepsis, require prompt and precise identification of the causative bacteria for appropriate antibiotics treatment. Traditional methods such as culturing take 2-5 days, while newer techniques such as reverse transcription-polymerase chain reaction and mass spectrometry are hindered by blood impurities. Consequently, this study developed a surface-enhanced Raman scattering (SERS)-based acoustofluidic technique for rapid bacterial detection without culturing or lysing. Target bacteria are first tagged with SERS nanotags in a microtube. The solution with tagged bacteria and unbound SERS nanotags is passed through a silicon microfluidic channel. A piezoelectric transducer generates acoustic waves within the channel, concentrating larger tagged bacteria in the center and pushing smaller unbound nanotags toward the channel walls. A laser beam is focused at the center of the channel, and the Raman signals of bacteria passing through the focal volume are measured for quantitative analysis. As a proof of concept, this study detected various concentrations of at a limit of detection of 1.75 × 10 CFU/mL within 1 h. This method offers significant clinical potential, enabling rapid and accurate bacterial identification without genetic material extraction, cultivation, or lysis.
细菌感染，如败血症，需要迅速准确地鉴定出致病细菌以便进行适当的抗生素治疗。传统方法如培养需要2至5天，而诸如逆转录聚合酶链反应和质谱分析等较新技术则受到血液杂质的阻碍。因此，本研究开发了一种基于表面增强拉曼散射（SERS）的声流体技术，用于无需培养或裂解即可快速检测细菌。首先在微管中用SERS纳米标签标记目标细菌。含有标记细菌和未结合SERS纳米标签的溶液通过硅微流体通道。压电换能器在通道内产生声波，将较大的标记细菌集中在中心，并将较小的未结合纳米标签推向通道壁。激光束聚焦在通道中心，测量通过焦点体积的细菌的拉曼信号以进行定量分析。作为概念验证，本研究在1小时内检测到各种浓度的[细菌名称未给出]，检测限为1.75×10 CFU/mL。该方法具有巨大的临床潜力，能够在不进行遗传物质提取、培养或裂解的情况下快速准确地鉴定细菌。
