Zhou Yanhong, Zhang Wei, Cheng Shaowen, Xing Yanlong, Wang Juan, Yu Fabiao, Wang Rui
Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan Trauma and Disaster Rescue, Key Laboratory of Haikou Trauma, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, 571199, China; Engineering Research Center for Hainan Bio-Smart Materials and Bio-Medical Devices, Key Laboratory of Hainan Functional Materials and Molecular Imaging, College of Emergency and Trauma, Hainan Medical University, Haikou, 571199, China.
Key Laboratory of Tropical Translational Medicine, Ministry of Education, Key Laboratory of Brain Science Research and Transformation in Tropical Environment of Hainan Province, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, 571199, China.
Talanta. 2025 Oct 1;293:128089. doi: 10.1016/j.talanta.2025.128089. Epub 2025 Apr 3.
The early and accurate detection of pathogenic bacteria in infected wounds is crucial for preventing severe complications such as sepsis and multiple organ failure. This study presents a new surface-enhanced Raman scattering (SERS) platform based on polydimethylsiloxane (PDMS) flexible substrates and SERS probes for culture-free detection of Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Pseudomonas aeruginosa (P. aeruginosa). The PDMS flexible substrates, functionalized with gold nanoparticles (Au NPs) and specific antibodies, facilitate the efficient capture of bacteria and enhance the SERS signals through electromagnetic field coupling. The SERS probes, composed of Au@Ag core-shell nanoparticles with double-layer Raman reporter molecules, provide high sensitivity and specificity in bacterial detection. In the presence of target bacteria, the immunocomplex of PDMS flexible substrates, bacteria, and SERS probes forms, generating strong SERS signals and enabling sensitive bacterial detection. The SERS platform demonstrated limits of detection as low as 2.79 CFU/mL for E. coli, 3.42 CFU/mL for S. aureus, and 4.52 CFU/mL for P. aeruginosa. Additionally, the platform successfully monitored bacterial count changes in infected wound models and detected bacteria in clinical samples. These results indicate that the proposed SERS platform is a powerful tool for the rapid and sensitive detection of multiple bacteria in infected wounds, offering significant potential for clinical applications in infection management and advancing the field of bacterial diagnostics.
感染伤口中病原菌的早期准确检测对于预防败血症和多器官功能衰竭等严重并发症至关重要。本研究提出了一种基于聚二甲基硅氧烷（PDMS）柔性基底和表面增强拉曼散射（SERS）探针的新型平台，用于对大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌进行免培养检测。用金纳米颗粒（Au NPs）和特异性抗体功能化的PDMS柔性基底有助于高效捕获细菌，并通过电磁场耦合增强SERS信号。由具有双层拉曼报告分子的Au@Ag核壳纳米颗粒组成的SERS探针在细菌检测中具有高灵敏度和特异性。在存在目标细菌的情况下，形成了PDMS柔性基底、细菌和SERS探针的免疫复合物，产生强烈的SERS信号，从而实现灵敏的细菌检测。该SERS平台对大肠杆菌的检测限低至2.79 CFU/mL，对金黄色葡萄球菌为3.42 CFU/mL，对铜绿假单胞菌为4.52 CFU/mL。此外，该平台成功监测了感染伤口模型中的细菌数量变化，并检测了临床样本中的细菌。这些结果表明，所提出的SERS平台是一种用于快速灵敏检测感染伤口中多种细菌的强大工具，在感染管理的临床应用和推动细菌诊断领域方面具有巨大潜力。
