Mao Wuyu, Dong Ping, Du Wei, Wu Haoxing
Department of Respiratory and Critical Care Medicine, Institute of Respiratory Health, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Respiratory Health and Multimorbidity, West China Hospital of Sichuan University, Chengdu 610041, China.
Department of Nuclear Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Chem Biomed Imaging. 2024 Dec 23;3(1):1-4. doi: 10.1021/cbmi.4c00095. eCollection 2025 Jan 27.
A variety of bioorthogonal chemical tools have been developed and widely used in the study of biological phenomena in situ. Tetrazine bioorthogonal chemistry exhibits ultrafast reaction kinetics, excellent biocompatibility, and precise optical regulatory capabilities. Fluorogenic tetrazine bioorthogonal probes have achieved particularly diverse applications in bioimaging and disease diagnosis and treatment. This Viewpoint briefly introduces the characteristics and advantages of tetrazine bioorthogonal chemistry, some design strategies of fluorogenic tetrazine probes, and the status of applications of these tools to in vivo imaging, as well as disease diagnosis and treatment. Finally, we discuss challenges and propose future trends in the field of fluorogenic tetrazine probes. This Viewpoint offers insights into the development of new bioorthogonal tools for chemical biology research and for the design of new drugs.
多种生物正交化学工具已被开发并广泛应用于原位生物现象的研究。四嗪生物正交化学具有超快的反应动力学、优异的生物相容性和精确的光学调控能力。荧光四嗪生物正交探针在生物成像以及疾病诊断与治疗方面已实现了特别多样化的应用。本观点简要介绍了四嗪生物正交化学的特点和优势、荧光四嗪探针的一些设计策略，以及这些工具在体内成像、疾病诊断与治疗中的应用现状。最后，我们讨论了荧光四嗪探针领域面临的挑战并提出了未来趋势。本观点为化学生物学研究新型生物正交工具的开发以及新药设计提供了见解。
