Nasseri Seyed Amirhossein, Bains Rajneesh K, Tian Yuqing, Withers Stephen G
Department of Chemistry, University of British Columbia Vancouver Canada
Michael Smith Laboratories, University of British Columbia Vancouver Canada.
Chem Sci. 2025 Jan 28;16(8):3755-3757. doi: 10.1039/d4sc07749k. eCollection 2025 Feb 19.
We challenge the conclusion that the β-glucosidase in question directly catalyses hydrolysis of the substrate ester linkage. Rather we propose that this enzyme performs a normal glucoside hydrolysis and that the released aglycone undergoes rearrangement with formation of a quinone methide-like species through spontaneous cleavage of the ester.
我们对关于所讨论的β-葡萄糖苷酶直接催化底物酯键水解的结论提出质疑。相反,我们认为该酶进行正常的糖苷水解,并且释放出的苷元通过酯的自发裂解发生重排,形成类似醌甲基化物的物种。