Błaszkowski Janusz, Zubek Szymon, Milczarski Paweł, Malinowski Ryszard, Niezgoda Piotr, Goto Bruno Tomio
Department of Environmental Management, West Pomeranian University of Technology in Szczecin, Słowackiego 17, PL-71434 Szczecin, Poland.
Institute of Botany, Faculty of Biology, Jagiellonian University, 30-387, Kraków, Poland.
MycoKeys. 2025 Jan 22;112:253-276. doi: 10.3897/mycokeys.112.136158. eCollection 2025.
This article presents the results of morphological studies, as well as comparisons and phylogenetic analyzes of sequences of four arbuscular mycorrhizal fungi (AMF, phylum Glomeromycota): , strain 211, Isolate 517, and Isolate 524. strain 211 was previously characterized only by sequences of the 45S nuc rDNA region (= 18S, partial, ITS-1-5.8S-ITS2, 28S, partial) and the gene (without any morphological data) that were deposited in GenBank under the incorrect name " strain 211". Its 45S sequences differed from the original sequences and, consequently, resulted in erroneous phylogenetic classification of this species. Isolate 517 and Isolate 524 slightly differed in morphology from (formerly ) and (formerly ), respectively. was originally found in a maritime dune site of Italy in 2009 and not yet reported from any other habitat in the world. Our sequence comparisons and analyses showed that represents a new genus, here created under the name with , and strain 211 is a new species, described as These analyses also indicated that Isolate 517 is conspecific to and confirmed the correctness of the transfer of by other AMF researchers to gen. nov. with comb. nov. Morphological studies of our specimens grown in culture showed that the original description of this species is incomplete and, therefore, the description was emended. Phylogenetic analyses of Isolate 524 proved its conspecificity to and thus revealed its second site of occurrence, i.e., the coastal dunes of the Hel Peninsula in northern Poland.
本文介绍了四种丛枝菌根真菌（AMF，球囊菌门）：菌株211、分离物517和分离物524的形态学研究结果，以及序列比较和系统发育分析。菌株211之前仅通过45S核rDNA区域（=18S，部分，ITS-1-5.8S-ITS2，28S，部分）和基因序列（无任何形态学数据）进行了表征，这些序列以错误的名称“菌株211”存入GenBank。其45S序列与原始序列不同，因此导致该物种的系统发育分类错误。分离物517和分离物524在形态上分别与（原）和（原）略有不同。最初于2009年在意大利的一个海岸沙丘地点发现，世界上其他任何栖息地都未报告过。我们的序列比较和分析表明，代表一个新属，在此以名称创建，并且菌株211是一个新物种，被描述为这些分析还表明分离物517与同种，并证实了其他AMF研究人员将转移到新属并新组合的正确性。我们在培养物中生长的标本的形态学研究表明，该物种的原始描述不完整，因此对描述进行了修订。对分离物524的系统发育分析证明了它与同种，从而揭示了它的第二个出现地点，即波兰北部海尔半岛的海岸沙丘。
