埃塞俄比亚西北部贡德尔中部固体废弃物倾倒场中重金属抗性细菌的分离、筛选与特性分析
Isolation, screening, and characterization of heavy metal-resistant bacteria from solid waste dumping sites in Central Gondar, Northwest, Ethiopia.
作者信息
Dinku Abebech, Jiru Tamene Milkessa
机构信息
Department of Environmental and Industrial Biotechnology, Institute of Biotechnology, University of Gondar, Gondar, Ethiopia.
出版信息
Sci Prog. 2025 Jan-Mar;108(1):368504251315807. doi: 10.1177/00368504251315807.
OBJECTIVE
Heavy metal pollution is one of the more recent problems of environmental degradation caused by rapid industrialization and human activity. The objective of this study was to isolate, screen, and characterize heavy metal-resistant bacteria from solid waste disposal sites.
METHODS
In this study, a total of 18 soil samples were randomly selected from mechanical sites, metal workshops, and agricultural land that received wastewater irrigation. Isolation and screening of the isolates were based on multiple heavy metal (copper (Cu), chromium (Cr), and lead (Pb)) resistance potential. Morphological, biochemical, and 16S rRNA gene sequencing techniques were used to identify the isolates. The minimum inhibitory concentration (MIC) of the potential isolates was further examined at various concentrations (3, 6, 12, 25, 50, and 100 µg/mL). Each isolate's ability to biodegrade and resist antibiotics was also examined.
RESULTS
About 21 bacterial isolates were obtained. Among these, six potential isolates (Agri10Is2, Agri10Is2, Agri10Is1, AL10Is2, AL10Is1, and GA10Is1) were selected. The isolates displayed varied colony morphologies and biochemical characterization features. Phylogenetic tree results revealed that Agri10Is2, Agri10Is2, Agri10Is1, AL10Is2, AL10Is1, and GA10Is1 were identified as , , species, , species, and , respectively. Every isolate gave a different MIC value. The biodegradation capacity of Agri10Is2, Agri10Is2, Agri10Is1, AL10Is2, AL10Is1, and GA10Is1 was 92.2%, 92.57%, 92.37%, 92.66%, 92.85%, and 92.52% against Cu; 95.07%, 94.07%, 93.07%, 93.4%, 91.4%, and 94.4% on Cr; and 95.51%, 94.53%, 95.62%, 96.87%, 94.86%, and 97.09% against Pb, respectively. All isolates were found to be resistant to the tested antibiotics.
CONCLUSION
This study concludes that the selected bacterial isolates could be potential agents for bioremediation of heavy metal-polluted environments.
目的
重金属污染是快速工业化和人类活动导致的较新的环境退化问题之一。本研究的目的是从固体废物处理场分离、筛选和鉴定抗重金属细菌。
方法
在本研究中，从接受废水灌溉的机械场地、金属加工车间和农田中随机选取了18个土壤样本。根据对多种重金属（铜（Cu）、铬（Cr）和铅（Pb））的抗性潜力对分离株进行分离和筛选。采用形态学、生化和16S rRNA基因测序技术对分离株进行鉴定。在不同浓度（3、6、12、25、50和100μg/mL）下进一步检测潜在分离株的最低抑菌浓度（MIC）。还检测了每个分离株的生物降解能力和抗生素抗性。
结果
共获得约21株细菌分离株。其中，筛选出6株潜在分离株（Agri10Is2、Agri10Is2、Agri10Is1、AL10Is2、AL10Is1和GA10Is1）。这些分离株表现出不同的菌落形态和生化特征。系统发育树结果显示，Agri10Is2、Agri10Is2、Agri10Is1、AL10Is2、AL10Is1和GA10Is1分别被鉴定为 种、 种和 种。每个分离株的MIC值不同。Agri10Is2、Agri10Is2、Agri10Is1、AL10Is2、AL10Is1和GA10Is1对Cu的生物降解能力分别为92.2%、92.57%、92.37%、92.66%、92.85%和92.52%；对Cr的生物降解能力分别为95.07%、94.07%、93.07%、93.4%、91.4%和94.4%；对Pb的生物降解能力分别为95.51%、94.53%、95.62%、96.87%、94.86%和97.09%。所有分离株均对测试抗生素具有抗性。
结论
本研究得出结论，所选细菌分离株可能是重金属污染环境生物修复的潜在 agents。 （注：原文中“agents”未明确翻译，可能是“媒介、制剂”等意思，此处保留原文未译完整的情况）
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