探究脂肪组织在活动能力和衰老过程中的作用：肌肉、活动能力与衰老研究附属脂肪组织研究（SOMMA-AT）的设计与方法

Investigating the role of adipose tissue in mobility and aging: design and methods of the Adipose Tissue ancillary to the Study of Muscle, Mobility, and Aging (SOMMA-AT).

作者信息

Yeo Reichelle X, Mau Theresa, Ross Zana M, Edenhoffer Nicholas P, Liu Jingfang, Barnes Haley N, Lui Li-Yung, Adkins Joshua N, Sanford James A, Seldin Marcus M, Viesi Carlos H, Zhou Mingqi, Gregory Heather L, Toledo Frederico G S, Stefanovic-Racic Maja, Lyles Mary, Wood Ashlee N, Mattila Polly E, Blakley Elizabeth A, Miljkovic Iva, Cawthon Peggy M, Newman Anne B, Kritchevsky Stephen B, Cummings Steven R, Goodpaster Bret H, Justice Jamie N, Kershaw Erin E, Sparks Lauren M

机构信息

AdventHealth Translational Research Institute, Orlando, Florida, USA.

Department of Epidemiology and Biostatistics, University of California, San Francisco, San Francisco, California, USA.

出版信息

J Gerontol A Biol Sci Med Sci. 2025 May 5;80(6). doi: 10.1093/gerona/glaf015.

DOI:10.1093/gerona/glaf015

PMID:39886989

Abstract

BACKGROUND

Age-related changes in adipose tissue affect chronic medical diseases and mobility disability but mechanism remains poorly understood. The goal of this study is to define methods for phenotyping unique characteristics of adipose tissue from older adults.

METHODS

Older adults enrolled in study of muscle, mobility, and aging selected for the adipose tissue ancillary (SOMMA-AT; N = 210, 52.38% women, 76.12 ± 4.37 years) were assessed for regional adiposity by whole-body magnetic resonance (AMRA) and underwent a needle-aspiration biopsy of abdominal subcutaneous adipose tissue (ASAT). ASAT biopsies were flash frozen, fixed, or processed for downstream applications and deposited at the biorepository. Biopsy yields, qualitative features, adipocyte sizes, and concentration of adipokines secreted in ASAT explant conditioned media were measured. Inter-measure Spearman correlations were determined.

RESULTS

Regional, but not total, adiposity differed by sex: women had greater ASAT mass (8.20 ± 2.73 kg, p < .001) and biopsy yield (3.44 ± 1.81 g, p < .001) than men (ASAT = 5.95 ± 2.30 kg, biopsy = 2.30 ± 1.40 g). ASAT mass correlated with leptin (r = 0.54, p < .001) and not resistin (p = .248) and adiponectin (p = .353). Adipocyte area correlated with ASAT mass (r = 0.34, p < .001), BMI (r = 0.33, p < .001), adiponectin (r = -0.22, p = .005) and leptin (r = 0.18, p = .024) but not with resistin (p = .490).

CONCLUSION

In addition to the detailed ASAT biopsy processing in this report, we found that adipocyte area correlated with ASAT mass, and both measures related to some key adipokines in the explant conditioned media. These results, methods, and biological repositories underscore the potential of this unique cohort to impact the understanding of aging adipose biology on disease, disability, and other aging tissues.

摘要

背景

脂肪组织的年龄相关变化会影响慢性疾病和行动能力障碍，但其机制仍知之甚少。本研究的目的是确定对老年人脂肪组织独特特征进行表型分析的方法。

方法

入选肌肉、行动能力与衰老研究附属脂肪组织研究（SOMMA-AT；N = 210，52.38%为女性，76.12 ± 4.37岁）的老年人，通过全身磁共振成像（AMRA）评估其局部脂肪含量，并对腹部皮下脂肪组织（ASAT）进行针吸活检。ASAT活检样本进行速冻、固定或处理以供下游应用，并存放于生物样本库。测量活检样本的产量、定性特征、脂肪细胞大小以及ASAT外植体条件培养基中分泌的脂肪因子浓度。确定各测量指标之间的斯皮尔曼相关性。

结果

局部脂肪含量存在性别差异，但总体脂肪含量无差异：女性的ASAT质量（8.20 ± 2.73千克，p <.001）和活检产量（3.44 ± 1.81克，p <.001）均高于男性（ASAT = 5.95 ± 2.30千克，活检产量 = 2.30 ± 1.40克）。ASAT质量与瘦素相关（r = 0.54，p <.001），与抵抗素（p =.248）和脂联素（p =.353）无关。脂肪细胞面积与ASAT质量（r = 0.34，p <.001）、体重指数（r = 0.33，p <.001）、脂联素（r = -0.22，p =.005）和瘦素（r = 0.18，p =.024）相关，但与抵抗素无关（p =.490）。