Mohammadi Mohammad Reza, Mohabbati Mobarez Ashraf, Broumand Mohammad Ali, Baseri Neda, Latifian Mina, Esmaeili Saber
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Pathology and Laboratory Medicine, Tehran Heart Center Tehran, University of Medical Sciences, Tehran, Iran.
Microbiol Spectr. 2025 Mar 4;13(3):e0185624. doi: 10.1128/spectrum.01856-24. Epub 2025 Jan 31.
The aim of this study was to investigate the prevalence of and in valve samples from endocarditis patients using the real-time PCR method at a major referral heart hospital in Iran. In this study, 146 paraffin-embedded tissue samples from the heart valves of patients with clinical and pathological evidence of infective endocarditis (IE), who underwent heart valve replacement surgery between 2016 and 2020 at Tehran Heart Center were collected. After DNA extraction from paraffin-embedded valve tissue samples, they were surveyed for the presence of and using quantitative real-time PCR (qPCR). The 16S rRNA gene sequence analysis was used for accurate species identification. Based on the molecular results, , spp., and spp. were detected in 10 (6.84%), 6 (4.1%), and 3 (2.05%) valve samples, respectively. In addition, one of six positive samples for spp. was identified as . No positive samples for and were found. In addition, all control valve samples were negative for all investigated pathogens. The findings suggest that specific bacterial species such as , and . are associated with the development of IE. Considering the life-threatening nature of IE, it is critical for healthcare systems to prioritize the identification of its causative agents and develop targeted treatment strategies.IMPORTANCEInfective endocarditis (IE) is a serious and potentially life-threatening condition, and it is associated with significant morbidity, mortality, and complications, making it a major concern in both global and national healthcare systems. Late diagnosis and failure to receive appropriate treatment for patients with culture-negative endocarditis caused by hard-to-grow bacteria can lead to the death of patients. Unfortunately, in Iran, less attention is paid to the role of organisms that are difficult to cultivate in laboratory settings such as and in causing culture-negative endocarditis, and these pathogens are overlooked by infectious, cardiologists, and health officials. This study underscores the need for special attention in the diagnosis of the agents of IE.
本研究的目的是在伊朗一家主要的心脏专科医院，采用实时荧光定量聚合酶链反应（PCR）方法，调查感染性心内膜炎患者瓣膜样本中[具体细菌名称1]和[具体细菌名称2]的流行情况。在本研究中，收集了2016年至2020年期间在德黑兰心脏中心接受心脏瓣膜置换手术、具有感染性心内膜炎（IE）临床和病理证据的患者的146份石蜡包埋组织样本。从石蜡包埋的瓣膜组织样本中提取DNA后，采用定量实时荧光定量聚合酶链反应（qPCR）检测其中[具体细菌名称1]和[具体细菌名称2]的存在情况。使用16S rRNA基因序列分析进行准确的菌种鉴定。根据分子检测结果，分别在10份（6.84%）、6份（4.1%）和3份（2.05%）瓣膜样本中检测到[具体细菌名称1]、[具体细菌名称3]属和[具体细菌名称4]属。此外，6份[具体细菌名称3]属阳性样本中的1份被鉴定为[具体细菌名称5]。未发现[具体细菌名称6]和[具体细菌名称7]的阳性样本。此外，所有对照瓣膜样本中所有调查的病原体均为阴性。研究结果表明，特定细菌种类如[具体细菌名称1]、[具体细菌名称3]和[具体细菌名称4]与IE的发生有关。考虑到IE的危及生命的性质，医疗系统优先识别其病原体并制定针对性治疗策略至关重要。
重要性
感染性心内膜炎（IE）是一种严重且可能危及生命的疾病，它与显著的发病率、死亡率和并发症相关，使其成为全球和国家医疗系统的主要关注点。对于由难以培养的细菌引起的培养阴性心内膜炎患者，诊断延迟和未能接受适当治疗可能导致患者死亡。不幸的是，在伊朗，对于在实验室环境中难以培养的微生物如[具体细菌名称1]和[具体细菌名称2]在引起培养阴性心内膜炎中的作用关注较少，这些病原体被感染科医生、心脏病专家和卫生官员忽视。本研究强调了在IE病原体诊断中需要特别关注。
