Bal Ipek, Balci Nur, Sorguc Cem, Toygar Hilal Uslu, Serdar Ceyhan C, Kurgan Sivge, Serdar Muhittin A
Department of Periodontology, Institute of Health Sciences, Istanbul Medipol University, Istanbul, Turkey.
Department of Periodontology, Faculty of Dentistry, Istanbul Medipol University, Istanbul, Turkey.
Oral Dis. 2025 Jan 30. doi: 10.1111/odi.15262.
Trimethylamine N-oxide (TMAO) is a compound involved in the pathogenesis of various systemic inflammatory diseases, including cardiovascular conditions. The aim of this study was to determine differences in saliva and serum levels of TMAO between periodontitis and healthy patients according to smoking status.
The study included four systemically healthy groups: periodontally healthy non-smokers (NS-Control; n = 25), non-smokers with Stage-III-Grade-B periodontitis (NS-Periodontitis; n = 25), periodontally healthy smokers (S-Control; n = 25), and smokers with Stage-III Grade-C periodontitis (S-Periodontitis; n = 25). Periodontal parameters were recorded. TMAO levels were determined in saliva and serum samples using liquid chromatography-mass spectrometry (LC-MS/MS). TNF-α levels were measured by the ELISA method.
Salivary TNF-α and TMAO levels were significantly elevated in the smoking periodontitis group compared to other groups (p < 0.001 and p = 0.003, respectively). Serum TMAO levels were also significantly higher in the smoking periodontitis group compared to non-smoking controls and non-smoking periodontitis. TMAO/SFR ratios were notably higher in the smoking periodontitis group compared to other groups, and a strong positive correlation was observed between salivary TMAO and TNF-α levels (r = 0.892, p < 0.001).
The data suggested that TMAO and TNF-α are associated with inflammatory mechanisms of periodontitis in cases where periodontitis coexists with smoking.
NCT06580431.
氧化三甲胺（TMAO）是一种参与包括心血管疾病在内的各种全身性炎症性疾病发病机制的化合物。本研究的目的是根据吸烟状况确定牙周炎患者与健康患者唾液和血清中TMAO水平的差异。
该研究纳入了四个全身健康的组：牙周健康的非吸烟者（非吸烟对照组；n = 25）、患有III级B期牙周炎的非吸烟者（非吸烟牙周炎组；n = 25）、牙周健康的吸烟者（吸烟对照组；n = 25）以及患有III级C期牙周炎的吸烟者（吸烟牙周炎组；n = 25）。记录牙周参数。使用液相色谱-质谱联用仪（LC-MS/MS）测定唾液和血清样本中的TMAO水平。采用酶联免疫吸附测定法（ELISA）测量肿瘤坏死因子-α（TNF-α）水平。
与其他组相比，吸烟牙周炎组的唾液TNF-α和TMAO水平显著升高（分别为p < 0.001和p = 0.003）。与非吸烟对照组和非吸烟牙周炎患者相比，吸烟牙周炎组的血清TMAO水平也显著更高。与其他组相比，吸烟牙周炎组的TMAO/肌酐清除率（SFR）比值明显更高，并且唾液TMAO与TNF-α水平之间存在强正相关（r = 0.892，p < 0.001）。
数据表明，在牙周炎与吸烟并存的情况下，TMAO和TNF-α与牙周炎的炎症机制相关。
NCT06580431。
