Yu Ting, Li Yang, Li Jing, Gan Yabing, Long Zhengze, Deng Yun, Zhang Youyu, Li Haitao, Yin Peng, Yao Shouzhuo
Institute of Interdisciplinary Studies, Hunan Normal University, Changsha, 410081, China.
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China.
Adv Sci (Weinh). 2025 Mar;12(11):e2415882. doi: 10.1002/advs.202415882. Epub 2025 Jan 30.
Adenosine triphosphate (ATP) is a critical intracellular energy currency that plays a key role in various cellular processes and is closely associated with numerous diseases. Similarly, biothiols such as glutathione (GSH), cysteine (Cys), and homocysteine (Hcy) are integral to many physiological and pathological processes due to their strong redox properties. Simultaneous discrimination and detection of ATP and biothiols offer valuable insights into the pathogenesis of conditions such as epilepsy and liver injury. This study introduces the first fluorescent probe, BCR, designed for multifunctional detection of ATP, GSH, Hcy, and Cys. With outstanding optical properties, excellent biocompatibility, high selectivity, and superior sensitivity, probe BCR enables effective imaging of ATP and biothiol dynamics in vivo. Moreover, probe BCR successfully visualizes changes in ATP, GSH, Hcy, and Cys levels in a PTZ-induced epileptic zebrafish model and an APAP-induced mouse liver injury tissue section model. These findings underscore the significant potential of probe BCR for early disease diagnosis and therapeutic applications.
三磷酸腺苷（ATP）是一种关键的细胞内能量货币，在各种细胞过程中发挥着关键作用，并且与多种疾病密切相关。同样，生物硫醇如谷胱甘肽（GSH）、半胱氨酸（Cys）和同型半胱氨酸（Hcy）因其强大的氧化还原特性，对许多生理和病理过程不可或缺。同时鉴别和检测ATP与生物硫醇，为深入了解癫痫和肝损伤等病症的发病机制提供了有价值的见解。本研究介绍了首个用于多功能检测ATP、GSH、Hcy和Cys的荧光探针BCR。探针BCR具有出色的光学性质、优异的生物相容性、高选择性和卓越的灵敏度，能够在体内对ATP和生物硫醇动态进行有效成像。此外，探针BCR成功地在戊四氮诱导的癫痫斑马鱼模型和对乙酰氨基酚诱导的小鼠肝损伤组织切片模型中，可视化了ATP、GSH、Hcy和Cys水平的变化。这些发现凸显了探针BCR在疾病早期诊断和治疗应用方面的巨大潜力。
