肌球蛋白在离体肠上皮刷状缘终末网收缩中的作用。
Role of myosin in terminal web contraction in isolated intestinal epithelial brush borders.
作者信息
Keller T C, Conzelman K A, Chasan R, Mooseker M S
出版信息
J Cell Biol. 1985 May;100(5):1647-55. doi: 10.1083/jcb.100.5.1647.
Abstract
We have investigated the role of myosin in contraction of the terminal web in brush borders isolated from intestinal epithelium. At 37 degrees C under conditions that stimulate terminal web contraction (1 microM Ca++ and ATP), most (60-70%) of the myosin is released from the brush border. Approximately 80% of the myosin is also released by ATP at 0 degree C, in the absence of contraction. Preextraction of this 80% of the myosin from brush borders with ATP has no effect on either the time course or extent of subsequently stimulated contraction. However, contraction is inhibited by removal of all of the myosin with 0.6 M KCl and ATP. Contraction is also inhibited by an antibody to brush border myosin, which inhibits both the ATPase activity of brush border myosin and its ability to form stable bipolar polymers. These results indicate that although functional myosin is absolutely required for terminal web contraction only approximately 20% of the brush border myosin is actually necessary. This raises the possibility that there are at least two different subsets of myosin in the terminal web.
摘要
我们研究了肌球蛋白在从肠上皮分离的刷状缘终末网收缩中的作用。在37摄氏度、刺激终末网收缩的条件下（1微摩尔钙离子和ATP），大部分（60%-70%）的肌球蛋白从刷状缘释放。在0摄氏度、无收缩的情况下，约80%的肌球蛋白也会被ATP释放。用ATP从刷状缘预先提取这80%的肌球蛋白，对随后刺激收缩的时间进程或程度均无影响。然而，用0.6M氯化钾和ATP去除所有肌球蛋白会抑制收缩。抗刷状缘肌球蛋白抗体也会抑制收缩，该抗体既能抑制刷状缘肌球蛋白的ATP酶活性，又能抑制其形成稳定双极聚合物的能力。这些结果表明，虽然终末网收缩绝对需要功能性肌球蛋白，但实际上刷状缘肌球蛋白中只有约20%是必需的。这增加了终末网中至少存在两种不同肌球蛋白亚群的可能性。
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