Zhang Yaxing, Cai Zhiwei, Zou Rong, Wang Ruling, Tan Runan, Wang Lei, Wu Yuxiang, He Hanping, He Yunbin, Chang Gang
Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science and Engineering, Hubei University, Wuhan 430062, China.
College of Health Science and Engineering, Hubei University, Wuhan 430062, China.
ACS Sens. 2025 Feb 28;10(2):1187-1196. doi: 10.1021/acssensors.4c03073. Epub 2025 Jan 31.
Epithelial cell adhesion molecule (EpCAM) was considered to be an important marker of multiple tumors, and its high expression is closely related to the early diagnosis and treatment of tumors. At present, metal oxide semiconductors have become a key component of biosensor and bioelectronics technology. Tin oxide shows great potential for development because of its nontoxic, nonpolluting, low price, and excellent electrical properties. In this study, a novel SnO solution-gated thin film transistor (SGTFT) biosensor for the specific detection of EpCAM was successfully developed using SnO film prepared by the sol-gel method as the channel material. By selecting the optimal thickness of 100 nm SnO film as the channel material, the transconductance value () reached 1432 μS, and the threshold voltage () remained stable at 0.288 V. In order to achieve qualitative and quantitative detection of EpCAM, SnO films were subjected to a specific chemical treatment to fix the aptamer. Through a specific recognition between the aptamer and EpCAM, the gate voltage changes were triggered to regulate the channel current of the device. FE-SEM, EIS, XPS, and electrical performance tests were employed to track and measure the modification process. Based on the optimizations described above, the prepared SGTFT exhibited high detection sensitivity (14.6 mV·dec), the limit of detection (LOD) down to 24.4 pg/mL, and the calibration curves in the range of 0.02 ng/mL-500 ng/mL for EpCAM sensing. The developed SnO-SGTFT biosensor is anticipated to provide a new highly sensitive and specific detection platform for health monitoring and disease diagnosis.
上皮细胞粘附分子（EpCAM）被认为是多种肿瘤的重要标志物，其高表达与肿瘤的早期诊断和治疗密切相关。目前，金属氧化物半导体已成为生物传感器和生物电子技术的关键组成部分。氧化锡因其无毒、无污染、价格低廉和优异的电学性能而具有巨大的发展潜力。在本研究中，以溶胶-凝胶法制备的SnO薄膜为沟道材料，成功开发了一种用于特异性检测EpCAM的新型SnO溶液栅控薄膜晶体管（SGTFT）生物传感器。通过选择100 nm厚的SnO薄膜作为沟道材料的最佳厚度，跨导值（）达到1432 μS，阈值电压（）稳定在0.288 V。为了实现对EpCAM的定性和定量检测，对SnO薄膜进行了特定的化学处理以固定适配体。通过适配体与EpCAM之间的特异性识别，触发栅极电压变化以调节器件的沟道电流。采用场发射扫描电子显微镜（FE-SEM）、电化学阻抗谱（EIS）、X射线光电子能谱（XPS）和电学性能测试来跟踪和测量修饰过程。基于上述优化，制备的SGTFT表现出高检测灵敏度（14.6 mV·dec），检测限（LOD）低至24.4 pg/mL，以及在0.02 ng/mL - 500 ng/mL范围内用于EpCAM传感的校准曲线。所开发的SnO - SGTFT生物传感器有望为健康监测和疾病诊断提供一个新的高灵敏度和特异性的检测平台。
