Reeve J R, Ho F J, Walsh J H, Ben-Avram C M, Shively J E
J Chromatogr. 1985 Mar 15;321(2):421-32. doi: 10.1016/s0021-9673(01)90460-1.
Canine motilin has been purified from small amounts of canine intestine in a form suitable for microsequence analysis. The sequence determined is: Phe-Val-Pro-Ile-Phe-Thr-His-Ser-Glu-Leu-Gln-Lys-Ile-Arg-Glu-Lys-Glu-Arg- Asn-Lys - Ile-Arg-Asn-Lys-Gly-Gln. Canine motilin differs from porcine motilin at five positions. The rapid, high-yield (24% overall yield) microisolation techniques used for canine motilin should be suitable for the isolation of other basic peptides found in low levels in tissue that is available only in limited amounts. These methods should make the isolation and sequence determination of human brain and gut peptides more readily achievable.
犬胃动素已从少量犬肠道中纯化出来，其形式适合进行微量序列分析。所确定的序列为：苯丙氨酸-缬氨酸-脯氨酸-异亮氨酸-苯丙氨酸-苏氨酸-组氨酸-丝氨酸-谷氨酸-亮氨酸-谷氨酰胺-赖氨酸-异亮氨酸-精氨酸-谷氨酸-赖氨酸-谷氨酸-精氨酸-天冬酰胺-赖氨酸-异亮氨酸-精氨酸-天冬酰胺-赖氨酸-甘氨酸-谷氨酰胺。犬胃动素在五个位置上与猪胃动素不同。用于犬胃动素的快速、高产率（总产率24%）微量分离技术，应该适用于从仅能获得有限量的组织中低水平存在的其他碱性肽的分离。这些方法应该会使人类脑和肠道肽的分离及序列测定更容易实现。
