Gallagher R T, Hawkes A D
J Chromatogr. 1985 Mar 29;322(1):159-67. doi: 10.1016/s0021-9673(01)97668-x.
The lolitrem neurotoxins, potent tremorgenic toxins isolated from perennial ryegrass, were examined using high-performance liquid chromatography with stop-flow UV spectral characterization. Comparison with some known indoles and indolic tremorgenic mycotoxins, together with chemically reduced lolitrem B, the major lolitrem neurotoxin, established the central indole chromophore of the lolitrems. The stop-flow UV spectral characterization was useful for identification of lolitrem B in ryegrass plant and seed extracts.
利用高效液相色谱仪并结合停流紫外光谱表征技术，对从多年生黑麦草中分离出的强效震颤毒素——洛替毒素进行了研究。通过与一些已知的吲哚及吲哚类震颤性霉菌毒素进行比较，再加上对主要的洛替毒素——洛替毒素B进行化学还原，确定了洛替毒素的中心吲哚发色团。停流紫外光谱表征技术有助于鉴定黑麦草植株和种子提取物中的洛替毒素B。
