Gallagher R T, Hawkes A D, Stewart J M
J Chromatogr. 1985 Mar 8;321(1):217-26. doi: 10.1016/s0021-9673(01)90438-8.
A rapid, sensitive and quantitative method, based on high-performance liquid chromatography with fluorescence detection, is described for the determination of the neurotoxin lolitrem B in perennial ryegrass, in the ppm to sub-ppm range. The method, which requires a minimal clean-up step prior to chromatographic analysis, is suitable for the routine analysis of large numbers of ryegrass samples, and is currently being used in New Zealand to study the livestock disorder ryegrass staggers. The method is suitable for determining lolitrem B in the whole plant, the seed, and dissected plant components.
本文描述了一种基于高效液相色谱荧光检测的快速、灵敏且定量的方法，用于测定多年生黑麦草中百万分之一至亚百万分之一范围内的神经毒素洛替米B。该方法在色谱分析前只需进行最少的净化步骤，适用于大量黑麦草样品的常规分析，目前正在新西兰用于研究家畜疾病黑麦草蹒跚症。该方法适用于测定整株植物、种子及解剖后的植物组分中的洛替米B。
