Morrison L A, Driskell J A
J Chromatogr. 1985 Feb 8;337(2):249-58. doi: 10.1016/0378-4347(85)80038-4.
A rapid, sensitive procedure is described for the analysis of the B6 vitamers pyridoxal, pyridoxamine, and pyridoxine in human milk from women taking and not taking supplements containing the vitamin using high-performance liquid chromatography with fluorometric detection. Vitamer values represent the sum of their phosphorylated and unphosphorylated forms. Minimum detectable quantities were 1-3 ng. Excellent recoveries of these vitamers in milk were obtained. Similar B6 vitamer concentrations of milk were obtained using the developed high-performance liquid chromatographic and the accepted microbiological techniques. Pyridoxal, actually consisting of pyridoxal plus pyridoxal phosphate, was the predominant B6 vitamer in human milk. The concentration of B6 vitamers in milk was reflective of the maternal vitamin B6 status.
描述了一种快速、灵敏的方法，用于使用带荧光检测的高效液相色谱法分析服用和未服用含该维生素补充剂的女性母乳中的B6维生素（吡哆醛、吡哆胺和吡哆醇）。维生素值代表其磷酸化和未磷酸化形式的总和。最低检测量为1 - 3纳克。这些维生素在牛奶中的回收率极佳。使用开发的高效液相色谱法和公认的微生物技术获得了相似的母乳中B6维生素浓度。吡哆醛实际上由吡哆醛加磷酸吡哆醛组成，是母乳中主要的B6维生素。母乳中B6维生素的浓度反映了母亲的维生素B6状态。
