Manikandan Rajendran, Gollapalli Sivarama Krishna, Shreya Gopinath, Basagoudanavar Suresh, Sreenivasa B P, Dubey Samriddhi, Bhanuprakash V, Sanyal Aniket, Mahapatra Mana, Parida Satya, Hosamani Madhusudan
ICAR-Indian Veterinary Research Institute, Hebbal campus, Bengaluru, 560024, Karnataka, India.
ICAR-Indian Veterinary Research Institute, Hebbal campus, Bengaluru, 560024, Karnataka, India; ICAR-National Institute of High Security Animal Diseases, Bhopal, 462021, India.
Virology. 2025 Mar;604:110421. doi: 10.1016/j.virol.2025.110421. Epub 2025 Jan 23.
Foot-and-mouth disease (FMD) is a severe and, highly contagious viral disease caused by FMD virus (FMDV) that primarily targets ungulates. Identifying neutralising antigenic sites on the virus is crucial for understanding the virus's antigenic characteristics. Numerous studies have pinpointed the critical residues of the neutralising antigenic sites in FMDV serotypes O, A, and C. However, information on serotype Asia 1 is very limited. This study reports the generation and characterization of forty-one monoclonal antibodies (mAbs) against FMDV serotype Asia 1 (current Indian vaccine strain). Of these, a panel of 23 neutralising mAbs was utilized to generate mAb resistant (MAR) mutants for mapping the virus's antigenic sites. New critical residues defining sites 1, 2, and 4 in FMDV serotype Asia 1 were identified. These findings significantly enhance our understanding of the antigenic nature of FMDV serotype Asia 1, and provide insights to designing broadly acting vaccines against FMD.
口蹄疫（FMD）是一种由口蹄疫病毒（FMDV）引起的严重且高度传染性的病毒性疾病，主要感染有蹄类动物。确定病毒上的中和抗原位点对于了解病毒的抗原特性至关重要。众多研究已经指出了口蹄疫病毒O型、A型和C型中和抗原位点的关键残基。然而，关于亚洲1型的信息非常有限。本研究报告了针对口蹄疫病毒亚洲1型（当前印度疫苗株）的41种单克隆抗体（mAb）的产生和特性。其中，一组23种中和单克隆抗体被用于产生单克隆抗体抗性（MAR）突变体，以绘制病毒的抗原位点。确定了口蹄疫病毒亚洲1型中定义位点1、2和4的新关键残基。这些发现显著增强了我们对口蹄疫病毒亚洲1型抗原性质的理解，并为设计针对口蹄疫的广谱疫苗提供了见解。
