Liu Chang, Li Xuerong, Hu Qinan, Jia Zihan, Ye Qing, Wang Xianzhe, Zhao Kaichen, Liu Longqi, Wang Mingyue
BGI Research, Hangzhou 310030, China; BGI Research, Shenzhen 518083, China; Shanxi Medical University-BGI Collaborative Center for Future Medicine, Shanxi Medical University, Taiyuan 030001, China; Shenzhen Proof-of-Concept Center of Digital Cytopathology, BGI Research, Shenzhen 518083, China.
BGI Research, Hangzhou 310030, China.
Semin Cell Dev Biol. 2025 Mar;167:22-39. doi: 10.1016/j.semcdb.2025.01.002. Epub 2025 Jan 31.
Embryonic development is a complex and intricately regulated process that encompasses precise control over cell differentiation, morphogenesis, and the underlying gene expression changes. Recent years have witnessed a remarkable acceleration in the development of single-cell and spatial omic technologies, enabling high-throughput profiling of transcriptomic and other multi-omic information at the individual cell level. These innovations offer fresh and multifaceted perspectives for investigating the intricate cellular and molecular mechanisms that govern embryonic development. In this review, we provide an in-depth exploration of the latest technical advancements in single-cell and spatial multi-omic methodologies and compile a systematic catalog of their applications in the field of embryonic development. We deconstruct the research strategies employed by recent studies that leverage single-cell sequencing techniques and underscore the unique advantages of spatial transcriptomics. Furthermore, we delve into both the current applications, data analysis algorithms and the untapped potential of these technologies in advancing our understanding of embryonic development. With the continuous evolution of multi-omic technologies, we anticipate their widespread adoption and profound contributions to unraveling the intricate molecular foundations underpinning embryo development in the foreseeable future.
胚胎发育是一个复杂且受到精细调控的过程，它包括对细胞分化、形态发生以及潜在基因表达变化的精确控制。近年来，单细胞和空间组学技术的发展显著加速，使得在单个细胞水平上对转录组和其他多组学信息进行高通量分析成为可能。这些创新为研究调控胚胎发育的复杂细胞和分子机制提供了全新的、多方面的视角。在本综述中，我们深入探讨了单细胞和空间多组学方法的最新技术进展，并编制了它们在胚胎发育领域应用的系统目录。我们剖析了近期利用单细胞测序技术的研究所采用的研究策略，并强调了空间转录组学的独特优势。此外，我们深入探讨了这些技术目前的应用、数据分析算法以及在推进我们对胚胎发育理解方面尚未开发的潜力。随着多组学技术的不断发展，我们预计在可预见的未来，它们将被广泛采用，并为揭示胚胎发育的复杂分子基础做出重大贡献。
