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1
Effect of anticoagulants on the chromogenic Limulus lysate assay for endotoxin.抗凝剂对鲎试剂显色法检测内毒素的影响。
J Clin Pathol. 1985 Apr;38(4):430-2. doi: 10.1136/jcp.38.4.430.
2
Assay of endotoxin in human plasma using immobilized histidine, Limulus amoebocyte lysate and chromogenic substrate.使用固定化组氨酸、鲎试剂和显色底物测定人血浆中的内毒素
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A micromethod for endotoxin assay in human plasma using Limulus amoebocyte lysate and a chromogenic substrate.一种使用鲎试剂和显色底物检测人血浆中内毒素的微量方法。
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A sensitive chromogenic Limulus amoebocyte lysate micro-assay for detection of endotoxin in human plasma and in water.一种用于检测人血浆和水中内毒素的灵敏显色鲎试剂微量分析法。
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Principles of a quantitative assay for bacterial endotoxins in blood that uses Limulus lysate and a chromogenic substrate.一种使用鲎试剂和显色底物对血液中细菌内毒素进行定量测定的方法的原理。
J Clin Microbiol. 1980 Nov;12(5):644-50. doi: 10.1128/jcm.12.5.644-650.1980.
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Optimization of detection of bacterial endotoxin in plasma with the Limulus test.利用鲎试剂法优化血浆中细菌内毒素的检测
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引用本文的文献

1
Endotoxemia: methods of detection and clinical correlates.内毒素血症：检测方法及临床关联
Clin Microbiol Rev. 1995 Apr;8(2):268-92. doi: 10.1128/CMR.8.2.268.

本文引用的文献

1
A bacterial disease of Limulus polyphemus.鲎的一种细菌性疾病。
Bull Johns Hopkins Hosp. 1956 May;98(5):325-51.
2
Detection of endotoxin in blood and other specimens by evaluation of photometrically registered LAL-reaction-kinetics in microtiter plates.通过评估微量滴定板中光检测记录的鲎试剂反应动力学来检测血液和其他标本中的内毒素。
Prog Clin Biol Res. 1982;93:385-92.
3
Limulus anti-LPS factor: an anticoagulant which inhibits the endotoxin mediated activation of Limulus coagulation system.鲎抗脂多糖因子：一种抗凝剂，可抑制内毒素介导的鲎凝血系统激活。
Biochem Biophys Res Commun. 1982 Mar 30;105(2):717-23. doi: 10.1016/0006-291x(82)91493-0.
4
Observations on the measurement and evaluation of endotoxemia by a quantitative limulus lysate microassay.利用定量鲎试剂微量分析法对内毒素血症进行测量与评估的观察
J Infect Dis. 1984 Dec;150(6):916-24. doi: 10.1093/infdis/150.6.916.
5
An improved chromogenic substrate endotoxin assay for clinical use.一种用于临床的改进型显色底物内毒素检测法。
J Clin Pathol. 1983 Oct;36(10):1145-9. doi: 10.1136/jcp.36.10.1145.
6
Detection of endotoxin in human blood and demonstration of an inhibitor.人体血液中内毒素的检测及一种抑制剂的证明
J Lab Clin Med. 1970 Jun;75(6):903-11.
7
The management of pulmonary embolic disease.肺栓塞疾病的管理
Am J Med Sci. 1970 Mar;259(3):157-63. doi: 10.1097/00000441-197003000-00001.
8
Inhibitory effect of heparin on the Limulus test for endotoxin.肝素对内毒素鲎试剂检测的抑制作用。
J Clin Microbiol. 1976 Aug;2(2):151.
9
Chromogenic substrates for horseshoe crab clotting enzyme. Its application for the assay of bacterial endotoxins.鲎凝血酶的显色底物。其在细菌内毒素检测中的应用。
Haemostasis. 1978;7(2-3):183-8. doi: 10.1159/000214260.

抗凝剂对鲎试剂显色法检测内毒素的影响。

Effect of anticoagulants on the chromogenic Limulus lysate assay for endotoxin.

作者信息

McConnell J S, Cohen J

出版信息

J Clin Pathol. 1985 Apr;38(4):430-2. doi: 10.1136/jcp.38.4.430.

DOI:10.1136/jcp.38.4.430
PMID:3988956
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC499171/
Abstract

We have examined the effects of anticoagulants on the chromogenic Limulus lysate assay for endotoxin. The results indicate that both heparin and CPD Adenine 1 have a striking dose related inhibitory effect on the assay. At concentrations of heparin as low as about 30 U/ml there was a 90% reduction in detectable endotoxin. The inhibitory effect of up to 100 U/ml of heparin could be neutralised by the addition of protamine sulphate. Anticoagulants appear to be an additional factor which can adversely affect the Limulus chromogenic assay for endotoxin. This further emphasises the need for alternative approaches to the measurement of endotoxin.

摘要

我们研究了抗凝剂对内毒素显色鲎试剂检测法的影响。结果表明，肝素和CPD腺嘌呤1对该检测法均有显著的剂量相关抑制作用。在肝素浓度低至约30 U/ml时，可检测到的内毒素减少了90%。添加硫酸鱼精蛋白可中和高达100 U/ml肝素的抑制作用。抗凝剂似乎是另一个会对内毒素鲎试剂显色检测产生不利影响的因素。这进一步强调了采用替代方法测量内毒素的必要性。