一种使用鲎试剂和显色底物检测人血浆中内毒素的微量方法。
A micromethod for endotoxin assay in human plasma using Limulus amoebocyte lysate and a chromogenic substrate.
作者信息
Piotrowicz B I, Watt I, Edlin S, McCartney A C
出版信息
Eur J Clin Microbiol. 1985 Feb;4(1):52-4. doi: 10.1007/BF02148661.
PMID:3987679
Abstract
A modified micromethod for endotoxin assay is reported which involved incubation of plasma extract with Limulus amoebocyte lysate for 30 min followed by incubation with chromogenic substrate S-2423 for another 8 min. Absorbance is measured in microtitre plates. The sensitivity is less than 5 pg/ml (0.058 Endotoxin Units/ml).
摘要
报道了一种改良的内毒素检测微量方法，该方法包括将血浆提取物与鲎变形细胞溶解物孵育30分钟，然后再与显色底物S-2423孵育8分钟。在微量滴定板中测量吸光度。灵敏度小于5 pg/ml（0.058内毒素单位/ml）。
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