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一种使用鲎试剂和显色底物检测人血浆中内毒素的微量方法。

A micromethod for endotoxin assay in human plasma using Limulus amoebocyte lysate and a chromogenic substrate.

作者信息

Piotrowicz B I, Watt I, Edlin S, McCartney A C

出版信息

Eur J Clin Microbiol. 1985 Feb;4(1):52-4. doi: 10.1007/BF02148661.

DOI:10.1007/BF02148661
PMID:3987679
Abstract

A modified micromethod for endotoxin assay is reported which involved incubation of plasma extract with Limulus amoebocyte lysate for 30 min followed by incubation with chromogenic substrate S-2423 for another 8 min. Absorbance is measured in microtitre plates. The sensitivity is less than 5 pg/ml (0.058 Endotoxin Units/ml).

摘要

报道了一种改良的内毒素检测微量方法，该方法包括将血浆提取物与鲎变形细胞溶解物孵育30分钟，然后再与显色底物S-2423孵育8分钟。在微量滴定板中测量吸光度。灵敏度小于5 pg/ml（0.058内毒素单位/ml）。

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引用本文的文献

1
Endotoxin testing revisited.再谈内毒素检测
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本文引用的文献

1
THE ROLE OF ENDOTOXIN IN THE EXTRACELLULAR COAGULATION OF LIMULUS BLOOD.内毒素在鲎血体外凝血中的作用。
Bull Johns Hopkins Hosp. 1964 Sep;115:265-74.
2
Distribution and clearance of circulating endotoxin.循环内毒素的分布与清除
J Clin Invest. 1963 Jan;42(1):79-87. doi: 10.1172/JCI104698.
3
Quantitative endotoxin determination in blood with a chromogenic substrate.采用显色底物法对血液中的内毒素进行定量测定。
Clin Chim Acta. 1981 Oct 8;116(1):63-8. doi: 10.1016/0009-8981(81)90169-8.
4
Quantitative measurement of endotoxin in human blood using synthetic chromogenic substrate for horseshoe crab clotting enzyme: a comparison of methods of blood sampling and treatment.
Thromb Res. 1981;23(1-2):51-7. doi: 10.1016/0049-3848(81)90237-1.
5
Quantitative determination of endotoxin in plasma by means of a new chromogenic peptide substrate. A methodological study.采用新型显色肽底物定量测定血浆中的内毒素。一项方法学研究。
Acta Chir Scand Suppl. 1982;509:55-8.
6
Endotoxaemia in septic shock: clinical and post mortem correlations.脓毒性休克中的内毒素血症：临床与尸检相关性
Intensive Care Med. 1983;9(3):117-22. doi: 10.1007/BF01772577.
7
An improved chromogenic substrate endotoxin assay for clinical use.一种用于临床的改进型显色底物内毒素检测法。
J Clin Pathol. 1983 Oct;36(10):1145-9. doi: 10.1136/jcp.36.10.1145.
8
Clearance of endotoxin by platelets: role in increasing the accuracy of the Limulus gelation test and in combating experimental endotoxemia.血小板对内毒素的清除作用：在提高鲎试剂凝胶试验准确性及对抗实验性内毒素血症中的作用。
Surgery. 1973 Aug;74(2):235-40.
9
A sensitive substrate for the clotting enzyme in horseshoe crab hemocytes.一种用于鲎血细胞中凝血酶的敏感底物。
J Biochem. 1977 May;81(5):1567-9.