Tang Junjie, Eltaweil Abdelazeem S, Adeyemi Adeleye S, Jacobson Astrid R, Britt David W, McLean Joan E, Su Yiming
Utah Water Research Laboratory, Utah State University, Logan, UT 84322, United States.
Department of Chemistry, Faculty of Science, Alexandria University, Alexandria 21321, Egypt.
J Hazard Mater. 2025 May 5;488:137357. doi: 10.1016/j.jhazmat.2025.137357. Epub 2025 Jan 27.
Nanoplastics (NPs) are increasingly prevalent in the environment, posing potential risks to agricultural systems and the food web. Despite this, currently it lacks comprehensive evaluation on NPs detection and quantification techniques, which is critical for quantitatively understanding the fate and transport of NPs. To address this gap, our study systematically assesses and compares advanced analytical tools for tracking different types of NPs (derived from both top-down and bottom-up approaches) from soil to plants. For identifying and quantifying NPs from environmental samples, pyrolysis - gas chromatography - mass spectrometry (Py-GC-MS) and confocal-Raman spectroscopy demonstrate promise. For laboratory study, inductively coupled plasma mass spectrometry (ICP-MS) along with metal doped NPs enables good sensitivity for tracking NPs in plant system. Our results demonstrated a substantial NPs internalization, 1.09 × 10 ¹ ¹ NPs per gram in shoots and 1.52 × 10 ¹ ¹ NPs per gram in roots, by wheat seedlings after five days of exposure, leading to a notable 77.26 % reduction in biomass. This study highlights the importance of integrating multiple techniques to overcome the limitations of each individual technique and provides quantitative insight into the detection of NPs within plant systems, contributing to the improvement of methodology for NPs related research in environmental and agricultural fields.
纳米塑料（NPs）在环境中日益普遍，对农业系统和食物网构成潜在风险。尽管如此，目前缺乏对纳米塑料检测和定量技术的全面评估，而这对于定量了解纳米塑料的归宿和迁移至关重要。为了填补这一空白，我们的研究系统地评估和比较了用于追踪从土壤到植物的不同类型纳米塑料（源自自上而下和自下而上方法）的先进分析工具。对于从环境样品中识别和定量纳米塑料，热解-气相色谱-质谱联用仪（Py-GC-MS）和共聚焦拉曼光谱显示出应用前景。对于实验室研究，电感耦合等离子体质谱（ICP-MS）结合金属掺杂纳米塑料能够很好地灵敏追踪植物系统中的纳米塑料。我们的结果表明，小麦幼苗在暴露五天后，纳米塑料大量内化，地上部分每克有1.09×10¹¹个纳米塑料，根部每克有1.52×10¹¹个纳米塑料，导致生物量显著减少77.26%。本研究强调了整合多种技术以克服每种技术局限性的重要性，并提供了对植物系统中纳米塑料检测的定量见解，有助于改进环境和农业领域与纳米塑料相关研究的方法。
