Destrez P, Beysens D
ASP - Advanced Sterilization Products, 33 Technology Drive, Irvine, CA, 92618, USA.
ESPCI, Physique et Mécanique des Milieux Hétérogènes, CNRS, ESPCI Paris-PSL University, Sorbonne Université, Sorbonne Paris Cité, 10 Rue Vauquelin, 75005, Paris, France; OPUR, Organisation pour l'Utilisation de la Rosée, 2 Rue Verderet, 70016, Paris, France.
Anal Biochem. 2025 Jun;701:115786. doi: 10.1016/j.ab.2025.115786. Epub 2025 Jan 29.
Gazeous hydrogen peroxide (HO) is commonly used for disinfection of room surfaces or sterilization of medical devices. Disinfection and sterilization processes are controlled by mean values measured at sterilizer chamber or room level. However, the surface phenomena (adsorption/condensation) taking place on inoculum are essential and still not well-known. In the present study, a solution of water and HO is sprayed in a room disinfection system (Glosair 400, ASP) for 12 min. Condensation mass, HO concentration, relative humidity (RH), macro zoom observations and inactivation kinetics of various microorganisms (Staphyloccocus aureus, Pseudomonas aeruginosa Candida albicans, Aspergillus niger) are reported. Macro-zoom observations reveal condensation and bubbling activities. Microbial inactivation is found optimal at low initial RH, corresponding to high HO vapor concentration and low condensed mass. HO concentration in the condensate is high and probably boosted by fractional condensation. In surface disinfection processes, inactivation of microorganisms occurs in presence of condensation although excessive condensation, due to high initial RH conditions or the presence of salt, decreases the microbial inactivation efficiency by dilution. While the present experimental conditions differ from those prevailing in HO sterilization (59 % HO under vacuum) or industrial disinfection processes (30 % HO at atmospheric pressure), they are partially transposable.
气态过氧化氢（HO）常用于房间表面消毒或医疗器械灭菌。消毒和灭菌过程由在灭菌器腔室或房间水平测量的平均值控制。然而，接种物上发生的表面现象（吸附/冷凝）至关重要且仍未得到充分了解。在本研究中，将水和HO的溶液在房间消毒系统（Glosair 400，ASP）中喷雾12分钟。报告了冷凝质量、HO浓度、相对湿度（RH）、宏观变焦观察结果以及各种微生物（金黄色葡萄球菌、铜绿假单胞菌、白色念珠菌、黑曲霉）的失活动力学。宏观变焦观察揭示了冷凝和冒泡活动。发现微生物失活在低初始RH时最佳，这对应于高HO蒸汽浓度和低冷凝质量。冷凝物中的HO浓度很高，可能是由分级冷凝促进的。在表面消毒过程中，微生物失活在有冷凝的情况下发生，尽管由于高初始RH条件或盐的存在导致的过度冷凝会通过稀释降低微生物失活效率。虽然目前的实验条件与HO灭菌（真空下59% HO）或工业消毒过程（大气压下30% HO）中普遍存在的条件不同，但它们部分是可转换的。
