Koide Rie, Nakashima Yoshie, Kojima Shohei, Sotomi Yohei, Sakata Yasushi, Sakata Yasuhiko, Ito Kaoru, Parrish Nicholas F
Genome Immunobiology RIKEN Hakubi Research Team, RIKEN Cluster for Pioneering Research and RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.
Laboratory for Developmental Genetics, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.
Virus Genes. 2025 Apr;61(2):239-243. doi: 10.1007/s11262-025-02134-6. Epub 2025 Jan 31.
Endogenous human herpesvirus 6 (eHHV-6) is integrated into the genomes of approximately 1% of individuals and has been linked to angina pectoris which, like myocardial infarction (MI), generally denotes coronary artery disease. To investigate this association further, we screened 2976 genomic DNA samples from patients with MI for eHHV-6 using multiplex qPCR. We identified 17 eHHV-6-positive individuals (0.57%), including 6 with eHHV-6A, 10 with eHHV-6B, and 1 with the solo-DR form, an eHHV-6B variant characterized by the presence of a single direct repeat (DR) within the host genome. While subjects with eHHV-6 had slightly higher total cholesterol levels, this difference was not statistically significant after correction for multiple testing. Our large-scale screening demonstrates the prevalence of eHHV-6 in MI patients and highlights the potential of this approach for studying its impact on diseases and other complex traits.
内源性人类疱疹病毒6型（eHHV-6）整合到约1%个体的基因组中，并且与心绞痛有关，心绞痛与心肌梗死（MI）一样，通常意味着冠状动脉疾病。为了进一步研究这种关联，我们使用多重定量聚合酶链反应（qPCR）对2976例心肌梗死患者的基因组DNA样本进行了eHHV-6筛查。我们鉴定出17例eHHV-6阳性个体（0.57%），其中6例为eHHV-6A，10例为eHHV-6B，1例为单-DR形式，这是一种eHHV-6B变体，其特征是在宿主基因组中存在单个直接重复序列（DR）。虽然eHHV-6阳性的受试者总胆固醇水平略高，但在进行多重检验校正后，这种差异没有统计学意义。我们的大规模筛查证明了eHHV-6在心肌梗死患者中的流行情况，并突出了这种方法在研究其对疾病和其他复杂性状影响方面的潜力。
