香蒲花粉提取物通过α-MSH/MC1R信号通路抑制B16和黄褐斑小鼠模型中的黑色素生成。
Typha pollen extract inhibit melanogenesis via α-MSH/MC1R signaling pathway in B16 and melasma mouse model.
作者信息
Zheng Shiqian, Deng Rongrong, Xie Shengjun, Huang Gengjiu, Ou Zhiwen, Shen Zhibin
机构信息
Guangdong Botanical Beauty Care Biotechnology Co., Ltd, Guangzhou, China.
School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
出版信息
Arch Dermatol Res. 2025 Jan 31;317(1):321. doi: 10.1007/s00403-024-03752-0.
The aim of this study was to investigate the effects of Typha Pollen extract on melanogenesis in B16 and melasma mouse model. The effects of TP extract on B16 viability, melanin content of B16, MC1R content and TYR activity were studied by MTT assay, NaOH method, ELISA assay and dopa oxidation assay, respectively. In melasma mouse model, the effects of TP extract on MC1R content, MITF content and TYR activity were studied by ELISA assay, immunofluorescence staining and dopa oxidation assay, respectively. The results showed that the appropriate concentration of TP extract does not affect cell viability, and that TP extract inhibit B16 melanin, MC1R content and TYR activity. The results showed that TP extract inhibit skin MC1R content, MITF content and TYR activity. TP extract inhibit B16 melanin, inhibit proteins of α-MSH/MC1R signaling pathway such as MC1R, MITF and TYR. So TP extract is a natural and effective potential whitening agent.
本研究旨在探讨香蒲花粉提取物对B16和黄褐斑小鼠模型黑色素生成的影响。分别采用MTT法、NaOH法、ELISA法和多巴氧化法研究了TP提取物对B16细胞活力、B16黑色素含量、MC1R含量和TYR活性的影响。在黄褐斑小鼠模型中，分别采用ELISA法、免疫荧光染色法和多巴氧化法研究了TP提取物对MC1R含量、MITF含量和TYR活性的影响。结果表明，适当浓度的TP提取物不影响细胞活力，且TP提取物可抑制B16黑色素、MC1R含量和TYR活性。结果表明，TP提取物可抑制皮肤MC1R含量、MITF含量和TYR活性。TP提取物可抑制B16黑色素，抑制α-MSH/MC1R信号通路的蛋白如MC1R、MITF和TYR。因此，TP提取物是一种天然有效的潜在美白剂。
Zotero 插件